Conditioned Medium from Tonsil-Derived Mesenchymal Stem Cells Relieves CCl4 -Induced Liver Fibrosis in Mice

Tissue Engineering and Regenerative Medicine
Yu-Hee KimKyung-Ha Ryu

Abstract

The liver is an organ with remarkable regenerative capacity; however, once chronic fibrosis occurs, liver failure follows, with high mortality and morbidity rates. Continuous exposure to proinflammatory stimuli exaggerates the pathological process of liver failure; therefore, immune modulation is a potential strategy to treat liver fibrosis. Mesenchymal stem cells (MSCs) with tissue regenerative and immunomodulatory potential may support the development of therapeutics for liver fibrosis. Here, we induced hepatic injury in mice by injecting carbon tetrachloride (CCl4) and investigated the therapeutic potential of conditioned medium from tonsil-derived MSCs (T-MSC CM). In parallel, we used recombinant human IL-1Ra, which, as we have previously shown, is secreted exclusively from T-MSCs and resolves the fibrogenic activation of myoblasts. Hepatic inflammation and fibrosis were determined by histological analyses using H&E and Picro-Sirius Red staining. The results demonstrated that T-MSC CM treatment significantly reduced inflammation as well as fibrosis in the CCl4-injured mouse liver. IL-1Ra injection showed effects similar to T-MSC CM treatment, suggesting that T-MSC CM may exert anti-inflammatory and anti-fibrotic effects via...Continue Reading

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Citations

Dec 22, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jieun KimYoungmi Jung
Sep 4, 2020·Tissue Engineering and Regenerative Medicine·Ying-Hsien KaoCheuk-Kwan Sun

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