Conditioned medium of demineralized freeze-dried bone activates gene expression in periodontal fibroblasts in vitro

Journal of Periodontology
Guenther Schuldt FilhoReinhard Gruber

Abstract

Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native bone chips can activate transforming growth factor (TGF)-β signaling in mesenchymal cells. The aim of this study is to determine whether processing of native bone into DBM affects the activity of the conditioned medium. Porcine cortical bone blocks were subjected to defatting, different concentrations of hydrochloric acid, and various temperatures. DBM was lyophilized, ground, and placed into culture medium. Human gingiva and periodontal fibroblasts were exposed to the respective conditioned medium obtained from DBM (DBCM). Changes in the expression of TGF-β target genes were determined. DBCM altered the expression of TGF-β target genes (e.g., adrenomedullin, pentraxin 3, KN motif and ankyrin repeat domains 4, interleukin 11, NADPH oxidase 4, and BTB [POZ] domain containing 11) by at least five-fold. The response was observed in fibroblasts from both sources. Defatting lowered the activity of DBCM. The TGF-β receptor type I kinase inhibitor SB431542 [4-(4-(benzo[d][1,3]dioxol-5-yl)-5-(pyridin-2-yl)-1H-imidazol-2-yl)benzamide] but not the inhibitor of bone morphogenetic protein receptor dorsomorphin, blocked the effects o...Continue Reading

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Citations

Jan 29, 2016·Clinical Oral Implants Research·Jordi Caballé-SerranoReinhard Gruber
Mar 11, 2015·Clinical Oral Implants Research·Jordi Caballé-SerranoReinhard Gruber
Sep 27, 2016·Journal of Oral Science·Kosaku SawadaReinhard Gruber
Jan 31, 2018·The Journal of Craniofacial Surgery·Babak Hatami KiaReinhard Gruber
Mar 1, 2019·Clinical Implant Dentistry and Related Research·Franz Josef StraussReinhard Gruber
Oct 19, 2016·The Journal of Craniofacial Surgery·Branko HarnikReinhard Gruber
Feb 4, 2016·Implant Dentistry·José Moisés de Souza JúniorMarco Aurélio Bianchini
Sep 2, 2020·Clinical Oral Investigations·Ludovica ParisiMaria B Asparuhova
Oct 31, 2018·Scientific Reports·Franz Josef StraussReinhard Gruber
Jun 14, 2018·International Journal of Oral Science·Maria B AsparuhovaVivianne Chappuis
May 27, 2021·Plastic and Reconstructive Surgery. Global Open·David G ArmstrongCharles M Zelen

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