Conditioned Medium of Wharton's Jelly Derived Stem Cells Can Enhance the Cartilage Specific Genes Expression by Chondrocytes in Monolayer and Mass Culture Systems

Advanced Pharmaceutical Bulletin
Maryam Hassan FamianAzadeh Montaseri

Abstract

Purpose: Mesenchymal stem cells (MSCs) have been introduced for cell therapy strategies in osteoarthritis (OA). Despite of their capacity for differentiation into chondrocyte, there are some evidences about their life-threatening problem after transplantation. So, some researchers shifted on the application of stem cells conditioned medium. The goal of this study is to evaluate whether Wharton's jelly derived stem cell conditioned medium (WJSCs-CM) can enhance the gene expression profile by chondrocytes in monolayer and mass culture systems. Methods: Conditioned medium was obtained from WJSCs at fourth passage. Isolated chondrocytes were plated at density of 1×106 for both monolayer and high density culture. Then cells in both groups were divided into control (received medium) and experiment group treated with WJ-CM for 3 and 6 days. Samples were prepared to evaluate gene expression profile of collagen II, aggrecan, cartilage oligomeric matrix protein (COMP) and sox-9 using real-time RT-PCR. Results: After 3 days, Chondrocytes treated with WJSCs-CM expressed significantly higher level of genes compared to the control group in both culture systems. After 6 days, the expression of genes in monolayer cultivated chondrocytes w...Continue Reading

Citations

Aug 26, 2017·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
Aug 18, 2020·Biomedical Research·Wataru AriyoshiTatsuji Nishihara
Feb 24, 2021·International Journal of Biological Macromolecules·Ezzatollah FathiBehnaz Valipour
Jun 3, 2021·Materials·Aziz EftekhariMohammad Hasanzadeh
Jun 20, 2021·The Indian Journal of Medical Research·Vindya Lankika UdalamaththaPreethi Vidya Udagama

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BETA
PCR
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