PMID: 987016Jul 1, 1976

Conditions affecting primary cell cultures of functional adult rat hepatocytes. 1. The effect of insulin

In Vitro
B A Laishes, G M Williams

Abstract

The conditions for obtaining representative, primary adult rat hepatocyte cultures were explored. The methods applied included enzymatic liver perfusion which was nondestructive to hepatocytes, the prevention of aggregation of dissociated cells and the selective attachment of viable cells. These procedures yielded a recovery of 50% of the liver cells which gave rise to cultures representing 14% of the total liver cells. The cultures were composed of homogeneous epithelial-like cells cytologically similar to hepatocytes and possessed a number of liver-specific enzymes. There was virtually no cell division initially and most cells died between 24 and 48 hr. Insulin enhanced the attachment of the liver cells, altered their morphology, but did not prolong cell survival.

References

Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·P BiberfeldM Raftell
Mar 1, 1972·The Journal of Cell Biology·H L Leffert, D Paul
Apr 28, 1972·Biochemical and Biophysical Research Communications·W R Ingebretsen, S R Wagle
Aug 6, 1973·Biochemical and Biophysical Research Communications·S R WagleL Sampson
Nov 1, 1974·Experimental Cell Research·G M Williams, J M Gunn
Nov 1, 1974·Experimental Cell Research·J Alwen, A M Lawn
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M E Kaighn, A M Prince
Nov 1, 1971·Experimental Cell Research·G M WilliamsJ H Weisburger
Nov 25, 1968·Biochemical and Biophysical Research Communications·L E Gerschenson, D Casanello
May 1, 1957·British Medical Bulletin·R D HARKNESS
Nov 1, 1947·The American Journal of Anatomy·S H WEBSTERD J ZIMMER

Citations

Mar 25, 1994·Biotechnology and Bioengineering·B D FoyM L Yarmush
Jan 1, 1985·Cell Biology and Toxicology·J J BermanG M Williams
Aug 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·G N Pluzhnikova, E S Ianakopulu
Jan 1, 1987·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M MiyazakiJ Sato
Jan 1, 1984·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M MiyazakiJ Sato
Oct 1, 1981·In Vitro·J E KlaunigB F Trump
Oct 1, 1981·In Vitro·J E KlaunigB F Trump
Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D AcostaM A Smith
Oct 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K Wirthensohn, C A Barth
May 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L AgiusK G Alberti
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S A Suleiman, J B Stevens
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K FurukawaG M Williams
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P Manos, D Holten
Jan 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M MiyazakiJ Sato
Sep 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M MiyazakiJ Sato
Apr 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N TakahashiN Mori
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M Pilar LópezJ V Castell
Feb 1, 1985·Mechanisms of Ageing and Development·C J Maslansky, G M Williams
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R LangenbachE Huberman
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A B KaneJ L Farber
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S Watanabe, M J Phillips
Mar 1, 1979·Journal of Toxicology and Environmental Health·P O Seglen
Mar 1, 1983·The Journal of Clinical Investigation·M ChelladuraiI Kushner
Aug 1, 1978·Environmental Health Perspectives·J W GrishamD G Kaufman
May 26, 1998·Environmental Health Perspectives·A Guillouzo
Sep 1, 1981·Journal of Toxicology and Environmental Health·C A McQueen, G M Williams
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B SaadP Maier
Jan 1, 1984·Tissue & Cell·R Jansing, W A Samsonoff
Jan 1, 1987·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·D WangH Sidransky
Feb 19, 1979·Biochimica Et Biophysica Acta·M L FaillaM J Mascenik
Jan 26, 1981·Biochimica Et Biophysica Acta·B P Schick, P K Schick
Mar 5, 2013·Expert Opinion on Drug Metabolism & Toxicology·Tom De BruynPieter Annaert
Oct 15, 1984·FEBS Letters·A P Wolffe, J R Tata
Aug 5, 2011·Journal of Bioscience and Bioengineering·Kevin MontagneYasuyuki Sakai
May 1, 1988·General and Comparative Endocrinology·C VaillantY Valotaire
Oct 15, 1978·Experimental Cell Research·J E Stanchfield, J D Yager
Dec 1, 1980·Biochemical Medicine·G V GnoniE Quagliariello
Jan 1, 1982·Annals of the New York Academy of Sciences·S S MacintyreI Kushner
Apr 1, 1984·Cell Biochemistry and Function·S OhkumaT Mukainaka
Nov 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M J PhillipsC R Smith
Jan 1, 1980·Annals of the New York Academy of Sciences·E BorenfreundA Bendich
Jan 1, 1980·Annals of the New York Academy of Sciences·J A Gurr, V R Potter
Jan 1, 1980·Annals of the New York Academy of Sciences·R MontesanoL Tomatis
Jan 1, 1980·Annals of the New York Academy of Sciences·D M Bissell, P S Guzelian
Mar 1, 1987·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·L R ShullJ A Wisniewski
Feb 1, 1978·Biochimica Et Biophysica Acta·M L Failla, R J Cousins
Jul 29, 1983·Biochemical and Biophysical Research Communications·I Leroux-NicolletN Marceau
Nov 1, 1980·Experimental Cell Research·P E Schwarze, P O Seglen
Jun 1, 1987·Cell Biology and Toxicology·D A Casciano
Dec 1, 1986·Journal of Medical Virology·Y ShimizuH Sasaki
Nov 1, 1982·The Anatomical Record·J E KlaunigB F Trump
Feb 15, 1978·International Journal of Cancer. Journal International Du Cancer·B A LaishesE Farber

Related Concepts

Metazoa
Cell Adhesion
Cell Division Phases
Cell Separation
Cell Survival
DNA, Double-Stranded
Insulin B Chain
Liver
Rats, Laboratory

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.