Nov 1, 1975

Conditions for splitting protodioscine--the main glycoside from Tribulus terrestris L. by the enzymatic preparation from Aspergillus niger BKMt-33

Prikladnaia biokhimiia i mikrobiologiia
E D PrepelitsaP K Kintya


The conditions for splitting protodioscine--the main steroid saponine isolated from Tribulus terrestris L. by the enzymic preparation of Aspergillus niger str. BKMt-33 were investigated. The optimal conditions were found to be as follows: pH 4-5, temperature 30-37 degrees (the substrate concentration--5 mg%, concentration of the enzymic preparation--1%). Under these conditions the enzymolysis continued 24 hours. Mg+2 and K+ ions accelerated the reaction twice. As a result of the enzymic hydrolysis dioscine and trilline were obtained. This indicates beta-glucosidase and alpha-rhamnosidase activities of the enzymic complex isolated from Aspergillus niger str. BKMt-33.

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Mentioned in this Paper

Tribulus terrestris
Cardiac Glycosides
Catatoxic Steroids
Aspergillus niger antigen
Tribulus terrestris extract
Aspergillus niger var. niger extract
Magnesium Cation

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