PMID: 11343956May 10, 2001Paper

Conditions of vector delivery improve efficiency of adenoviral-mediated gene transfer to the transplanted heart

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
John YapC G McGregor

Abstract

Conditions for ex vivo gene transfer to the transplanted heart were studied in a model of syngeneic abdominal heterotopic heart transplantation in the rat. Various methods of adenoviral-mediated gene transfer to the transplanted heart were compared. In the first experiment, a dose response study, an adenoviral vector encoding the beta-galactosidase gene was infused into the donor heart with the pulmonary artery open and flushed out prior to performing the transplant. In the second experiment, the effects of clamping the pulmonary artery during vector infusion and not flushing out the viral solution, resulting in vector dwell during the warm ischemia, were examined. In the first experiment, gene transfer was relatively inefficient; however, transgene expression improved with increases in the vector dose (range, 1x10(7)-1x10(9)). The efficiency of gene transfer was significantly greater when the conditions of the second experiment were applied. In all models studied, cardiomyocytes and not vascular endothelial cells were the predominant cell type transduced. This study indicates that the conditions of adenoviral vector delivery are critical for optimizing gene transfer in the transplant setting. In addition, intravascular adminis...Continue Reading

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Citations

Jun 19, 2010·Journal of Cardiovascular Translational Research·Justin A Mariani, David M Kaye
Nov 6, 2007·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Davide RicciChristopher G A McGregor
Feb 14, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gabor M Rubanyi
Apr 4, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Johannes M SchirmerChristopher G A McGregor
Dec 22, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Vinay P RaoChristopher G A McGregor
Apr 17, 2014·Journal of Cardiovascular Pharmacology·Gabor M Rubanyi

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