Conditions to improve expansion of human mesenchymal stem cells based on rat samples.

World Journal of Stem Cells
Maryam AyatollahiMohammad Hosein Sanati

Abstract

To improve the isolation and expansion of human marrow-derived mesenchymal stem cells (MSCs) based on rat samples. Based on the fact that rat MSCs are relatively easy to obtain from a small aspirate, bone marrow-derived MSCs from rat were cultured and characterized to set up the different protocols used in this study. Then, accordingly, almost the same protocols were performed on human healthy bone marrow samples, after obtaining approval of the ethics committee and gaining informed consent. We used different protocols and culture conditions, including the type of basal media and the culture composition. The MSCs were characterized by immunophenotyping and differentiation. There was no difference in morphology and proliferation capacity between different culture media at the first passage. During the 5-7th passages, the cells gradually lost their morphology and proliferation potential on Dulbecco's modified Eagle's medium (DMEM) high glucose and α modified Eagle's medium. Although the cells expanded rapidly for up to 10 passages on DMEM low glucose containing 10% to 15% fetal calf serum (FCS), their proliferation was arrested without change in morphology and differentiation capacity at the third passage on 5% FCS. Flow cytometr...Continue Reading

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Citations

Jul 6, 2014·Journal of Materials Science. Materials in Medicine·Alessandra ZamparelliRoberto Toni
Aug 12, 2015·Biotechnology Progress·Seyed Mohammad Ali Hosseini RadMajid Sadeghizadeh
Jan 1, 2016·Stem Cell Research & Therapy·Rhian StavelyKulmira Nurgali
Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·İsmail Alper İşoğluErhan Pişkin
Mar 13, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Nimet BölgenErhan Pişkin
Jun 9, 2020·Tissue Engineering and Regenerative Medicine·Dina M RostomEshrak A El Sabaawy

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