PMID: 7539844Mar 15, 1995Paper

Conductance and kinetics of single cGMP-activated channels in salamander rod outer segments

The Journal of Physiology
W R Taylor, D A Baylor

Abstract

1. The conductance and kinetics of single 3',5'-cyclic guanosine monophosphate (cGMP)-activated channels of retinal rod outer segments were studied in inside-out membrane patches. The size of the single channel currents was increased by using low concentrations of divalent cations. 2. At saturating cGMP concentration, the current flickered at high frequency. Occasionally, the current was interrupted by closures lasting tens or hundreds of milliseconds. At +50 mV the maximum current during an opening was slightly more than 1 pA, but the open channel level was poorly resolved due to the speed of the gating transitions. 3. Amplitude histograms confirmed the presence of a sublevel of current, roughly a quarter the size of the peak current, at low cGMP concentrations. The fraction of time in the sublevel decreased with increasing cGMP concentration, suggesting that the sublevel may be due to opening by the partially liganded channel. 4. Consistent with previous macroscopic current recordings, single channel activation by cGMP had an apparent dissociation constant of 8.6 microM, and a Hill coefficient of 2.8. 5. At saturating cGMP concentrations, the channel was modelled as a two-state system with the following parameters. The open c...Continue Reading

Citations

Jun 1, 1996·Journal of Bioenergetics and Biomembranes·W N Zagotta
Aug 5, 2000·The Journal of General Physiology·T R Middendorf, R W Aldrich
Jan 1, 1997·The Journal of General Physiology·A A FodorW N Zagotta
Aug 1, 1997·The Journal of General Physiology·S H Thompson
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Kimberly Matulef, William N Zagotta
Jul 27, 2005·The Journal of General Physiology·Mark L Chapman, Antonius M J VanDongen
Sep 5, 2001·Nature Reviews. Neuroscience·G E FlynnW N Zagotta
Nov 12, 2005·Pflügers Archiv : European journal of physiology·Mark L ChapmanAntonius M J VanDongen
Jun 28, 2002·Physiological Reviews·U Benjamin Kaupp, Reinhard Seifert
Jan 1, 2003·The Journal of General Physiology·Miguel Holmgren
Jun 1, 1995·Current Opinion in Neurobiology·A L Zimmerman
Jun 4, 2011·Journal of Medicinal Chemistry·Adriana L AndradeJeffrey W Karpen
Jun 11, 1998·Science·C RosenmundC F Stevens

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