Conductance studies on trichotoxin_A50E and implications for channel structure

Biophysical Journal
H DuclohierB A Wallace

Abstract

Trichotoxin_A50E is an 18-residue peptaibol whose crystal structure has recently been determined. In this study, the conductance properties of trichotoxin_A50E have been investigated in neutral planar lipid bilayers. The macroscopic current-voltage curves disclose a moderate voltage-sensitivity and the concentration-dependence suggests the channels are primarily hexameric. Under ion gradients, shifts of the reversal potential indicate that cations are preferentially transported. Trichotoxin displays only one single-channel conductance state in a given experiment, but an ensemble of experiments reveals a distribution of conductance levels. This contrasts with the related peptaibol alamethicin, which produces multiple channel levels in a single experiment, indicative of recruitment of additional monomers into different multimeric-sized channels. Based on these conductance measurements and on the recently available crystal structure of trichotoxin_A50E, which is a shorter and straighter helix than alamethicin, a tightly-packed hexameric model structure has been constructed for the trichotoxin channel. It has molecular dimensions and surface electrostatic potential compatible with the observed conductance properties of the most pro...Continue Reading

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Citations

Dec 17, 2014·The Journal of Chemical Physics·Michael A WilsonAndrew Pohorille
Jul 8, 2009·Research in Microbiology·Andrew Pohorille, David Deamer
Dec 13, 2016·The Journal of Physical Chemistry. B·Andrew PohorilleChenyu Wei
Jun 26, 2007·Chemistry & Biodiversity·Werner AltherrHeinz Heimgartner
Jun 26, 2007·Chemistry & Biodiversity·Thomas DegenkolbHans Brückner
Jun 26, 2007·Chemistry & Biodiversity·Hervé Duclohier
May 16, 2020·Journal of Fungi·Xiaoyan NiuQiongbo Hu
Mar 18, 2021·The Journal of Physical Chemistry. B·Andrew Pohorille, Michael A Wilson

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