PMID: 16529323Mar 15, 2006Paper

Conduction of injectable cartilage using fibrin sealant and human bone marrow mesenchymal stem cells in vivo

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Wei GeChun-hua Zhao

Abstract

To investigate the feasibility of the complex of the fibrin sealant (FS) and the bone marrow mesenchymal stem cells(MSCs) to create a new cartilage in the nude mice by the issue engineering technique. The MSCs were isolated from healthy humans and were expanded in vitro. And then the MSCs were induced by the defined medium containing the transforming growth factor beta1 (TGF-beta1), dexamethasone, and ascorbic acid. The biomechanical properties of the chondrocytes were investigated at 7 and 14 days. The MSCs induced for 7 days were collected and mixed with FS. Then, the FS-MSCs mixture was injected by a needle into the dorsum of the nude mice in the experimental group. In the two control groups, only FS or MSCs were injected respectively. The specimens were harvested at 6 and 12 weeks,and the ability of chondrogenesis in vivo was investigated by the gross observation, HE, Alcian Blue staining, and type II collagen immunohistochemistry. The MSCs changed from a spindle-like fibroblastic appearance to a polygonal shape when transferred to the defined medium, and could be induced to express the chondrocyte matrix. After an injection of the mixture, the cartilage-like tissue mass was formed, and the specimens were harvested from the...Continue Reading

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