PMID: 6985105Jan 1, 1982Paper

Cone lamellae and red and green rod outer segment disks contain a large intrinsic membrane protein on their margins: an ultrastructural immunocytochemical study of frog retinas

Vision Research
D S PapermasterB G Schneider

Abstract

In addition to rhodopsin, the disk membranes of rod outer segments (ROS) contain a large integral membrane protein (mol. wt 290,000). This protein was previously localized by immunocytochemistry to the margins and incisures of disks in frog red ROS by specific antibody applied to thin sections of bovine serum albumin embedded retinas (Papermaster et al., 1978b, J. Cell Biol. 78, 415-425). Upon further study of the reactions of this antibody with outer segments of other photoreceptor classes in frog retina, labeling of the short incisures and margins of green ROS and margins of cone outer segment lamellae is also observed. Thus the large protein participates in the structure of the edges of disks and lamellae of all photoreceptors in the frog. In addition, labeling of the inter-incisure surface of all photoreceptor classes was observed at high antibody concentration. In order to interpret this labeling, the effect of dilution on labeling density was determined and double reciprocal plots (Markham and Benton, 1931, J. Am. Chem. Soc. 53, 497) were employed to evaluate the relative affinity and heterogeneity. There was considerable deviation from linearity in the plots of labeling disk interiors compared to the relatively linear pl...Continue Reading

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Citations

Nov 13, 2007·Journal of Bioenergetics and Biomembranes·Robert S Molday
Mar 1, 1993·Biochimica Et Biophysica Acta·E N Pugh, T D Lamb
Jun 1, 1994·Insect Biochemistry and Molecular Biology·N KomoriH Matsumoto
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Jul 5, 2011·Biochemistry·Yaroslav TsybovskyKrzysztof Palczewski
Jan 1, 1988·Eye·A ShallalS al-Mahdawi
Nov 14, 1997·Nature Medicine·G H Travis, J Bennett
Mar 4, 2014·Protein Expression and Purification·Yaroslav Tsybovsky, Krzysztof Palczewski
Jul 17, 2010·Progress in Lipid Research·Robert S Molday, Kang Zhang
Aug 3, 2013·Chemical Reviews·Philip D KiserKrzysztof Palczewski
Feb 24, 2009·Biochimica Et Biophysica Acta·Robert S MoldayFaraz Quazi
May 23, 2006·Biophysical Journal·Giovanni CarusoEmmanuele DiBenedetto
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Faraz Quazi, Robert S Molday
Oct 10, 2007·Vision Research·Nora OverlackUwe Wolfrum
Nov 14, 2006·International Review of Cytology·Andrew F X Goldberg
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Sep 22, 1989·The Journal of Comparative Neurology·J M CorlessR D Fetter
Oct 10, 2021·Nature Communications·Jessica Fernandes ScortecciRobert S Molday

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