Configuration of bovine serum albumin adsorbed on polymer particles with grafted dextran corona

Colloids and Surfaces. B, Biointerfaces
C VauthierBernard Cabane

Abstract

The configuration of BSA macromolecules adsorbed on the surfaces of poly(alkylcyanoacrylate) nanoparticles has been determined using small angle neutron scattering (SANS). The nanoparticles were made by anionic emulsion polymerization (AEP) and self-assembly of dextran-poly(isobutylcyanoacrylate) (PICBA) copolymers. They have a hydrophobic PICBA core and a hydrophilic dextran corona. In vivo, they are recognized by the macrophages of the mononuclear phagocyte system. The amount of BSA bound to the particles, at adsorption equilibrium, has been determined through immunodiffusion, immunoelectrophoresis, and SANS. For particles with a radius of 25.3nm, the adsorption was found to saturate at 64 adsorbed BSA molecules per particle. The configuration of the adsorbed BSA molecules was determined from the SANS scattering curves, first at full contrast, and then at contrast match. Both experiments indicate that the BSA molecules are adsorbed on the PICBA core, in a flat configuration. This result may be important for understanding the in vivo opsonization mechanisms of nanoparticles and their resulting biodistribution.

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Citations

Jun 22, 2011·Chemical Reviews·Morteza MahmoudiSophie Laurent
Dec 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Andrew D MaynardMartin A Philbert
Jun 10, 2015·Drug Delivery·Jessy MariamPrabhakar M Dongre
Apr 3, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Khairallah AlharethFathi Moussa
Feb 22, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Khairallah AlharethFathi Moussa
Feb 26, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·M C B LiraC Vauthier
Jun 14, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cristina FornagueraChristine Vauthier
Apr 2, 2010·Journal of the American Chemical Society·Dorota WalczykKenneth A Dawson
Jan 19, 2021·ACS Biomaterials Science & Engineering·Xiaojun LongJun Lin
Oct 3, 2021·International Journal of Pharmaceutics·Christelle ZandanelChristine Vauthier

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