Configuration of PKCalpha-C2 domain bound to mixed SOPC/SOPS lipid monolayers.

Biophysical Journal
Chiu-Hao ChenMark L Schlossman

Abstract

X-ray reflectivity measurements are used to determine the configuration of the C2 domain of protein kinase Calpha (PKCalpha-C2) bound to a lipid monolayer of a 7:3 mixture of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine supported on a buffered aqueous solution. The reflectivity is analyzed in terms of the known crystallographic structure of PKCalpha-C2 and a slab model representation of the lipid layer. The configuration of lipid-bound PKCalpha-C2 is described by two angles that define its orientation, theta = 35 degrees +/- 10 degrees and phi =210 degrees +/- 30 degrees, and a penetration depth (=7.5 +/- 2 A) into the lipid layer. In this structure, the beta-sheets of PKCalpha-C2 are nearly perpendicular to the lipid layer and the domain penetrates into the headgroup region of the lipid layer, but not into the tailgroup region. This configuration of PKCalpha-C2 determined by our x-ray reflectivity is consistent with many previous findings, particularly mutational studies, and also provides what we believe is new molecular insight into the mechanism of PKCalpha enzyme activation. Our analysis method, which allows us to test all possible protein orientations, shows that our d...Continue Reading

References

Jan 27, 1998·The Journal of Biological Chemistry·O PerisicR L Williams
Oct 24, 2001·The Biochemical Journal·R V Stahelin, W Cho
Jan 24, 2002·Molecular Cell·Diana Murray, Barry Honig
Nov 12, 2002·The Journal of Biological Chemistry·Senena Corbalán-GarcíaJuan C Gómez-Fernández
Jan 15, 2003·The Journal of Biological Chemistry·Stephen R BolsoverSenena Corbalan-Garcia
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·David GidalevitzKa Yee C Lee
Dec 18, 2003·Science's STKE : Signal Transduction Knowledge Environment·Jonathan P DiNittoDavid G Lambright
Dec 31, 2003·Journal of Molecular Biology·Jose A Rodríguez-AlfaroSenena Corbalan-Garcia
May 5, 2005·Annual Review of Biophysics and Biomolecular Structure·Nathan J Malmberg, Joseph J Falke
May 5, 2005·Annual Review of Biophysics and Biomolecular Structure·Wonhwa Cho, Robert V Stahelin
Aug 5, 2005·The Journal of Biological Chemistry·Robert V StahelinWonhwa Cho
Nov 12, 2005·Clinical & Experimental Metastasis·Ying JiangYan Xu
Sep 5, 2006·Journal of Molecular Biology·Sonia Sánchez-BautistaSenena Corbalán-García
Jun 26, 2007·Journal of Molecular Biology·Marta Guerrero-ValeroSenena Corbalán-García
Jul 3, 2007·BMC Structural Biology·Andrei L LomizeHenry I Mosberg
Jan 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·M S KentS Satija
Feb 29, 2008·Journal of Molecular Biology·Consuelo Marín-VicenteSenena Corbalán-García

❮ Previous
Next ❯

Citations

May 25, 2012·The Journal of Membrane Biology·Jin-Won Park
May 15, 2013·Nature Structural & Molecular Biology·Alf HonigmannReinhard Jahn
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gregory T TietjenErin J Adams
Mar 29, 2014·Biochimica Et Biophysica Acta·Frank Heinrich, Mathias Lösche
Dec 15, 2010·Biochimica Et Biophysica Acta·Alessio AusiliDerek Marsh
Oct 24, 2012·Biochimica Et Biophysica Acta·Alessio AusiliJuan C Gómez-Fernández
Apr 22, 2015·Bioorganicheskaia khimiia·A A GlushkoM V Chernikov
Aug 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sebastian TiemeyerMetin Tolan
May 23, 2015·The Journal of Physical Chemistry. B·Javier L Baylon, Emad Tajkhorshid
Oct 8, 2014·The Journal of Physical Chemistry. B·Hao YuMark L Schlossman
May 3, 2011·Colloids and Surfaces. B, Biointerfaces·Jin-Won Park
May 23, 2012·Colloids and Surfaces. B, Biointerfaces·Jin-Won Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.