Configurational effects in antibody-antigen interactions studied by microcalorimetry

Proteins
K P MurphyY Paterson

Abstract

In this paper we study the binding of two monoclonal antibodies, E3 and E8, to cytochrome c using high-sensitivity isothermal titration calorimetry. We combine the calorimetric results with empirical calculations which relate changes in heat capacity to changes in entropy which arise from the hydrophobic effect. The change in heat capacity for binding E3 is -350 +/- 60 cal K-1 mol-1 while for E8 it is -165 +/- 40 cal K-1 mol-1. This result indicates that the hydrophobic effect makes a much larger contribution for E3 than for E8. Since the total entropy change at 25 degrees C is very similar for both antibodies, it follows that the configurational entropy cost for binding E3 is much larger than for binding E8 (-77 +/- 15 vs. -34 +/- 11 cal K-1 mol-1). These results illustrate a case of entropy compensation in which the cost of restricting conformational degrees of freedom is to a large extent compensated by solvent release. We also show that the thermodynamic data can be used to make estimates of the surface area changes that occur upon binding. The results of the present study are consistent with previous hydrogen-deuterium exchange data, detected using 2D NMR, on the two antibody-antigen interactions. The NMR study indicated t...Continue Reading

References

Sep 5, 1992·Journal of Molecular Biology·W R TulipP M Colman
Jan 1, 1992·Advances in Protein Chemistry·K P Murphy, E Freire
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T P Creamer, G D Rose
Dec 5, 1991·Journal of Molecular Biology·K P Murphy, S J Gill
Jan 1, 1990·Annual Review of Biochemistry·D R DaviesS Sheriff
Jan 1, 1985·Annual Review of Immunology·J A TainerR A Lerner
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S SheriffD R Davies
Jul 1, 1989·European Journal of Biochemistry·M OertleH R Bosshard
Jun 12, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P M ColmanR G Webster
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E A PadlanD R Davies
Oct 1, 1989·Protein Engineering·A V Finkelstein, J Janin
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S SpolarM T Record
Jan 1, 1988·Advances in Protein Chemistry·P L Privalov, S J Gill
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L Baldwin
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M I Page, W P Jencks
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·T N BhatR J Poljak
Jul 20, 1993·Journal of Molecular Biology·P L Privalov, G I Makhatadze
Jan 1, 1993·Progress in Biophysics and Molecular Biology·M Oobatake, T Ooi
Jun 5, 1993·Journal of Molecular Biology·S D Pickett, M J Sternberg
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S J Gill, I Wadsö

❮ Previous
Next ❯

Citations

Mar 1, 1996·Journal of Molecular Recognition : JMR·M L DoyleI M Chaiken
Mar 17, 1999·Journal of Molecular Recognition : JMR·J J VanAntwerp, K D Wittrup
Mar 1, 1996·Protein Science : a Publication of the Protein Society·G I Makhatadze, P L Privalov
May 1, 1996·Molecular Immunology·M Ohlin, C A Borrebaeck
Aug 1, 1995·Current Opinion in Structural Biology·B Nilsson
Feb 1, 1997·Current Opinion in Biotechnology·M L Doyle
Jan 27, 2009·Biochemistry·S MohanRichard C Willson
Nov 3, 2012·Protein Engineering, Design & Selection : PEDS·Takeshi NakanishiIzumi Kumagai
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michelle R ArkinAndrew C Braisted
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J J BonifaceM M Davis
May 17, 2006·Journal of Molecular Biology·Jakob DoganTorleif Härd
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·M M PierceB T Nall
Jul 16, 2016·Protein Science : a Publication of the Protein Society·Shigeki AraiRyota Kuroki
Apr 18, 2003·The Journal of Biological Chemistry·Kai HilpertWolfgang Höhne
Apr 23, 2008·Current Protocols in Protein Science·M L Doyle
Sep 4, 2004·Journal of Receptor and Signal Transduction Research·Remo PerozzoLeonardo Scapozza
Jun 8, 2006·Journal of the American Chemical Society·Elisabet Wahlberg, Torleif Härd
Sep 10, 2004·Journal of the American Chemical Society·Vildan Dincbas-RenqvistTorleif Härd

❮ Previous
Next ❯

Related Concepts

Related Feeds