Confirmation of reduced toxicity of deoxynivalenol in extrusion-processed corn grits by the MTT bioassay

Journal of Agricultural and Food Chemistry
Yuksel Cetin, Lloyd B Bullerman

Abstract

The objective of this study was to determine the loss of toxicity of deoxynivalenol in extruded cereal-based products by the tetrazolium salt (MTT) bioassay using a sensitive Chinese hamster ovary (CHO-K1) cell line and to compare the results to chemical (high-performance liquid chromatography, HPLC) and biochemical (enzyme-linked immunosorbant assay, ELISA) methods of analysis. A split-split plot design was used for the extrusion process experiments at temperatures of 150, 175, and 200 degrees C and screw speeds of 70 and 140 rpm. The initial mean deoxynivalenol concentration in the corn grits artificially contaminated with Fusarium graminearum was found to be 23.5 mug/g as measured by HPLC. The percent reductions of deoxynivalenol in the contaminated corn grits upon extrusion processing ranged from 22 to 35%, from 21 to 34%, and from 21 to 37% as measured by HPLC, ELISA, and MTT bioassay, respectively. The MTT bioassay results were more closely correlated with HPLC (r = 0.90) results than with ELISA results (r = 0.78). The MTT bioassay, using a sensitive mammalian cell line, was demonstrated to be a useful method for quantification of deoxynivalenol as well as a potential toxicity screening method for contaminated extruded ce...Continue Reading

References

Dec 1, 1994·Mycopathologia·M HaneltB Kollarczik
Jan 1, 1993·Food Additives and Contaminants·A E Pohland
May 1, 1996·Journal of Toxicology and Environmental Health·B A RotterJ J Pestka
Jun 17, 1997·International Journal of Food Microbiology·M S NeiraS L Resnik
May 21, 1999·Advances in Experimental Medicine and Biology·L S Jackson, L B Bullerman
Dec 22, 1999·Journal of Food Protection·M AccerbiP K Ng
Nov 25, 2000·Food Additives and Contaminants·G MoltoA Pacin
Jul 27, 2001·Letters in Applied Microbiology·D CazzanigaD M de Greef
Oct 25, 2002·Food Additives and Contaminants·R Elias-OrozcoG Loarca-Piña
Mar 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y Cetin, L B Bullerman
Aug 4, 2005·Journal of Agricultural and Food Chemistry·Yuksel Cetin, Lloyd B Bullerman
Jun 1, 1984·Journal of Food Protection·P M Scott

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Citations

Nov 23, 2007·Natural Product Reports·Braulio M Fraga
Jan 30, 2013·Colloids and Surfaces. B, Biointerfaces·Shangjing XinHongbing Deng
Feb 5, 2008·Journal of Microbiological Methods·Shamsozoha AbolmaaliGerhard Adam
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Sara Schaarschmidt, Carsten Fauhl-Hassek
May 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Sara Schaarschmidt, Carsten Fauhl-Hassek
Mar 18, 2021·Critical Reviews in Food Science and Nutrition·Ehsan Feizollahi, M S Roopesh
Apr 22, 2014·Analytical Chemistry·Suquan SongAibo Wu
Aug 17, 2006·Journal of Agricultural and Food Chemistry·Michael BretzHans-Ulrich Humpf

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