Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray.

Blood
Christine P HansWing C Chan

Abstract

Diffuse large B-cell lymphoma (DLBCL) can be divided into prognostically important subgroups with germinal center B-cell-like (GCB), activated B-cell-like (ABC), and type 3 gene expression profiles using a cDNA microarray. Tissue microarray (TMA) blocks were created from 152 cases of DLBCL, 142 of which had been successfully evaluated by cDNA microarray (75 GCB, 41 ABC, and 26 type 3). Sections were stained with antibodies to CD10, bcl-6, MUM1, FOXP1, cyclin D2, and bcl-2. Expression of bcl-6 (P <.001) or CD10 (P =.019) was associated with better overall survival (OS), whereas expression of MUM1 (P =.009) or cyclin D2 (P <.001) was associated with worse OS. Cases were subclassified using CD10, bcl-6, and MUM1 expression, and 64 cases (42%) were considered GCB and 88 cases (58%) non-GCB. The 5-year OS for the GCB group was 76% compared with only 34% for the non-GCB group (P <.001), which is similar to that reported using the cDNA microarray. Bcl-2 and cyclin D2 were adverse predictors in the non-GCB group. In multivariate analysis, a high International Prognostic Index score (3-5) and the non-GCB phenotype were independent adverse predictors (P <.0001). In summary, immunostains can be used to determine the GCB and non-GCB subtyp...Continue Reading

Citations

Jul 3, 2013·Cancer Immunology, Immunotherapy : CII·Bridget CharbonneauJames R Cerhan
Jul 4, 2012·Annals of Hematology·Byeong Seok SohnCheolwon Suh
Jun 19, 2013·Annals of Hematology·Armin Rashidi, Stephen I Fisher
Mar 8, 2011·Cancer Chemotherapy and Pharmacology·Martha RomeroAnne Janin
Dec 6, 2006·Der Pathologe·S M Kröber
Dec 17, 2009·Der Pathologe·S Hartmann, M L Hansmann
Oct 23, 2012·Der Pathologe·P AdamF Fend
Dec 21, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jiping CaiRuili Wei
Nov 2, 2004·Virchows Archiv : an International Journal of Pathology·Bettina FabianiUNKNOWN Groupe d'Etudes des Lymphomes de l'Adulte
Apr 14, 2007·Virchows Archiv : an International Journal of Pathology·Christoph LoddenkemperHarald Stein
Sep 23, 2008·Medical Molecular Morphology·Fumihiko KanekoToshifumi Hibi
Jun 1, 2011·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Hong-Wei ZhangWei Bai
Jun 1, 2006·Current Hematologic Malignancy Reports·Javeed IqbalKai Fu
Oct 1, 2007·Current Hematologic Malignancy Reports·Jane N Winter
Apr 29, 2010·Current Hematologic Malignancy Reports·Jason R Westin, Luis E Fayad
Apr 29, 2010·Current Hematologic Malignancy Reports·Peter McLaughlin
Jul 3, 2013·Current Hematologic Malignancy Reports·Jonas Lange, Birgit Burkhardt
Jul 31, 2013·Current Hematologic Malignancy Reports·Pei Lin, L Jeffrey Medeiros
Aug 5, 2004·Current Oncology Reports·Adam Bagg
Aug 12, 2006·Current Oncology Reports·Andrew J Davies
Aug 21, 2007·Current Oncology Reports·Ariela Noy
Jun 10, 2010·Head and Neck Pathology·Indraneel BhattacharyyaSamer Z Al-Quran
Mar 27, 2009·International Journal of Hematology·Yoshihiro KameokaKen-Ichi Sawada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Related Papers

The New England Journal of Medicine
Andreas RosenwaldLymphoma/Leukemia Molecular Profiling Project
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
William W L ChoiWing C Chan
The New England Journal of Medicine
International Non-Hodgkin's Lymphoma Prognostic Factors Project
Proceedings of the National Academy of Sciences of the United States of America
G WrightLouis M Staudt
© 2021 Meta ULC. All rights reserved