Confirmatory analysis of sulfonamide antibacterials in bovine liver and kidney: extraction with hot water and liquid chromatography coupled to a single- or triple-quadrupole mass spectrometer

Rapid Communications in Mass Spectrometry : RCM
Sara BogialliManuel Sergi

Abstract

A simple, specific, and rapid confirmatory method for determining 12 sulfonamide (SAs) antibacterials in bovine liver and kidney is presented. This method is based on the matrix solid-phase dispersion technique with hot water as extractant followed by liquid chromatography/mass spectrometry (LC/MS) with an electrospray ion source. The method was tailored for use with both single-quadrupole MS (I) and triple-quadrupole MS (II) instruments. After acidification and filtration of the aqueous extract, a 250-microL aliquot was injected into instrument I while only 25 microL was analyzed by instrument II. With instrument I MS data acquisition was performed in the selected ion monitoring (SIM) mode, selecting at least three ions for each target compound. With instrument II the selected reaction monitoring (SRM) mode with three fragmentation reactions for each compound was chosen. With the exception of sulfaquinoxaline (SQX), recovery of the analytes at the 50 ppb level in both liver and kidney was 72-96% with relative standard deviations (RSDs) ranging between 3 and 11%. The very poor recovery of SQX was due to its rapid enzymatic oxidation when in contact with the two tissues. With instrument I, limits of quantification (LOQs, S/N = 1...Continue Reading

Associated Clinical Trials

References

Aug 21, 1992·Science·H C Neu
Mar 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N A LittlefieldD W Gaylor
Jul 28, 1989·Journal of Chromatography·S A BarkerC R Short
Aug 15, 2000·Journal of Chromatography. a·S A Barker
Nov 1, 1996·Journal of the American Society for Mass Spectrometry·D L BuhrmanP J Rudewiczcor

❮ Previous
Next ❯

Citations

Feb 8, 2008·Analytical and Bioanalytical Chemistry·M García-LópezI Rodríguez
Nov 3, 2009·Analytical and Bioanalytical Chemistry·Consuelo Cháfer-PericásAmelia Moreno
Dec 3, 2013·Journal of Pharmaceutical and Biomedical Analysis·Rocío Cazorla-ReyesJosé Luis Martínez Vidal
Feb 3, 2012·Journal of Chromatographic Science·Yupu ZhangYinghua Wang
Aug 12, 2009·Journal of Chromatography. a·Bruno Le BizecJean-Philippe Antignac
Sep 25, 2003·Journal of Mass Spectrometry : JMS
Oct 31, 2003·Rapid Communications in Mass Spectrometry : RCM·Katerina KlagkouG John Langley
Aug 8, 2012·Drug Testing and Analysis·Rodrigo H M M GranjaAmarylis C B A Wanschel
Nov 28, 2006·Journal of Biochemical and Biophysical Methods·Sara Bogialli, Antonio Di Corcia
Nov 17, 2006·Journal of Biochemical and Biophysical Methods·Steven A Barker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.