Conflicting results between electrophoresis methods of serum M-proteins

B WuytsJ R Delanghe


The quantification of monoclonal immunoglobulins by protein electrophoresis has been helpful in determining the prognosis for the patient. In a 65-year old man with documented IgG lambda monoclonal gammopathy, a discrepancy in the detection and quantification of this M-protein was found when studied by four different electrophoretic methods. On a high-resolution agarose method with acid violet staining a prominent peak was seen in the fast gamma-region. A lower resolution agarose method using an amido black staining showed a small mid-gamma restriction, and no spike was detected by high-resolution or lower resolution capillary zone electrophoresis. The discrepancy was not attributable to a migration in the beta-region leading to masking by transferrin or C3 peak, incorrect separation on CZE due to a high isoelectric point (pI > 8.5) or pentamerization of IgM M-proteins. Therefore, other causes for discrepancies should be investigated.


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May 18, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Christof SchildJean-Marc Nuoffer
Jul 20, 2007·American Journal of Clinical Pathology·Zhaohai YangRobert W Hardy
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Nov 14, 2016·Clinical Biochemistry·Cecilia SartoPaolo Brambilla
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