Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning

Journal of Biomedical Optics
Thomas D WangGordon S Kino

Abstract

We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution < or =4.4 microm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.

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Citations

Sep 9, 2004·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Thomas D Wang, Jacques Van Dam
Aug 3, 2010·Nature Methods·Vasilis Ntziachristos
Nov 22, 2005·Nature Methods·Benjamin A FlusbergMark J Schnitzer
Jun 28, 2012·Journal of Biomedical Optics·Matthias C HofmannYong Xu
Jul 13, 2006·Annual Review of Biomedical Engineering·Vasilis Ntziachristos
Jul 4, 2012·Journal of Pathology Informatics·Yu ChenLiron Pantanowitz
Dec 3, 2008·The Review of Scientific Instruments·Elric EspositoGail McConnell
Nov 10, 2006·Journal of Biomedical Optics·Jonathan T C LiuThomas D Wang
Jul 4, 2008·Annals of the New York Academy of Sciences·Alberto BilencaGuillermo Tearney
Dec 13, 2005·Best Practice & Research. Clinical Gastroenterology·Louis-Michel Wong Kee Song, Brian C Wilson
Nov 4, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Steven SensarnChristopher H Contag
Jul 11, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Wafik S El-DeiryGary J Kelloff
Mar 16, 2007·Applied Optics·Ofer LeviJames S Harris
Mar 16, 2007·Applied Optics·Peter J DwyerMilind Rajadhyaksha

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