Confocal imaging of organic anion transport in intact rat choroid plexus

American Journal of Physiology. Renal Physiology
Christopher M BreenD S Miller

Abstract

We used confocal microscopy and quantitative image analysis to follow the movement of the fluorescent organic anion fluorescein (FL) from bath to cell and cell to blood vessel in intact rat lateral choroid plexus. FL accumulation in epithelial cells and underlying vessels was rapid, concentrative, and reduced by other organic anions. At steady state, cell fluorescence exceeded bath fluorescence by a factor of 3-5, and vessel fluorescence exceeded cell fluorescence by a factor of approximately 2. In cells, FL distributed between diffuse and punctate compartments. Cell and vessel accumulation of FL decreased when metabolism was inhibited by KCN, when bath Na(+) was reduced from 130 to 26 mM, and when the Na(+) gradient was collapsed with ouabain. Cell and vessel accumulation increased by >50% when 1-10 microM glutarate was added to the bath. Finally, transport of FL and carboxyfluorescein (generated intracellularly from carboxyfluorescein diacetate) from cell to blood vessel was greatly diminished when medium K(+) concentration ([K(+)]) was increased 10-fold. These results 1) validate a new approach to the study of choroid plexus function, and 2) indicate a two-step mechanism for transepithelial organic anion transport: indirect ...Continue Reading

References

Jan 5, 1979·Biochimica Et Biophysica Acta·S E BreslerN N Vasilieva
Mar 1, 1971·The American Journal of Physiology·H F Cserr, D H VanDyke
Jun 1, 1994·The American Journal of Physiology·J B PritchardD S Miller
Sep 1, 1994·The American Journal of Physiology·D S Miller, J B Pritchard
Oct 1, 1993·Physiological Reviews·J B Pritchard, D S Miller
May 1, 1993·The American Journal of Physiology·D S MillerJ B Pritchard
Sep 1, 1996·The American Journal of Physiology·D S MillerD M Barnes
Dec 1, 1996·The American Journal of Physiology·R MasereeuwD S Miller
Apr 13, 1999·The American Journal of Physiology·D S MillerJ B Pritchard
Jun 11, 1999·The American Journal of Physiology·A R VillalobosJ L Renfro
Nov 24, 1999·The Journal of Biological Chemistry·J B PritchardR Walden
Jun 3, 2000·American Journal of Physiology. Renal Physiology·G Burckhardt, N A Wolff
Aug 5, 2000·American Journal of Physiology. Renal Physiology·R A Van AubelF G Russel
Jan 3, 2001·Microscopy Research and Technique·B Gao, P J Meier

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Citations

Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Hiroyuki Kusuhara, Yuichi Sugiyama
Jul 25, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Valeska ReichelGert Fricker
May 18, 2010·Pharmaceutical Research·Reynold Spector, Conrad E Johanson
Jul 24, 2010·Journal of Neurochemistry·Valeska ReichelRosalinde Masereeuw
Dec 3, 2009·Biopharmaceutics & Drug Disposition·Adam L VanWertDouglas H Sweet
Mar 7, 2006·Free Radical Research·Kazuhiro IchikawaHideo Utsumi
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Maciej J Zamek-GliszczynskiKim L R Brouwer

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