Confocal laser scanning microscopy as a tool for imaging cancellous bone

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
I O SmithE D Case

Abstract

Understanding the bimodal structure of cancellous bone is important for tissue engineering in order to more accurately fabricate scaffolds to promote bone ingrowth and vascularization in newly forming bone. In this study, confocal laser scanning microscopy (CLSM) was used to create detailed images of the bimodally porous intertrabecular space of defatted and deproteinized cancellous canine bone taken from the epiphysis of the humerus. The bimodal pore structure was imaged using both reflective and fluorescent modes in CLSM, resulting in four different, but complementary image types: (1) a Z-stack overlay, (2) a phi-Z scan, (3) a topographical map, and (4) a contour map. Submerging the bone in rhodamine B dye prior to fluorescent imaging enhanced the pore surface details, giving a more accurate pore size measurement. The average macropore diameter was found to be 260 +/- 97 microm while the average micropore diameter was 13 +/- 10 microm. When compared with common techniques, including microcomputed tomography, magnetic resonance imaging, scanning electron microscopy, and environmental scanning electron microscopy, for imaging cancellous bone, CLSM was found to be an effective tool, given its ability to nondestructively image th...Continue Reading

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Citations

Oct 11, 2008·Tissue Engineering. Part B, Reviews·Irene GeorgakoudiDavid L Kaplan
Mar 2, 2010·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·J C RoldánG C Gurtner
Aug 24, 2010·Journal of Biomedical Materials Research. Part a·Michiyo HondaNobuyuki Kanzawa
May 12, 2010·Journal of Anatomy·Christoph PautkeThomas Tischer
Apr 24, 2015·Journal of Biomedical Materials Research. Part a·Vincent M J I CuijpersJohn A Jansen
Jul 6, 2010·Materials·Lutz-Christian Gerhardt, Aldo R Boccaccini

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