PMID: 11903803Mar 21, 2002Paper

Confocal laser scanning microscopy as a tool to determine cyanobacteria biomass in microbial mats

Journal of Microscopy
Antoni SoléIsabel Esteve

Abstract

We have developed a method based on confocal laser scanning microscopy for detection and quantification of cyanobacteria from the Ebro Delta microbial mats. Cyanobacteria play a major role as primary producers in microbial mats; it is difficult, however, to apply classical methods to estimate their biomass because they establish strong interactions with detritic particles. The protocol described here allows the localization of individual cells or filaments with micrometre precision without the need to either manipulate or stain the samples. This method is suitable for studying biomass 'in situ' in microbial mats.

Citations

Mar 21, 2021·Environmental Monitoring and Assessment·Carolina FernándezElisa R Parodi

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