Confocal laser scanning microscopy as an analytical tool in chromatographic research

Bioprocess and Biosystems Engineering
Jürgen Hubbuch, Maria Regina Kula

Abstract

In recent years, confocal laser scanning microscopy has been developed into a non-invasive tool to probe intra-particle profiles of protein in chromatographic adsorbents. A necessary prerequisite when using this technique lies in the labeling of proteins with fluorescent probes. The quality of the obtained results is thus strongly dependent on the probes used, its sensitivity on experimental parameters and the change of protein characteristics upon binding. In this review, the fundamental issues when using fluorescent probes are described, before giving a critical evaluation on published literature in the field of confocal laser scanning microscopy for the analysis of chromatographic principles.

References

Aug 13, 1998·Journal of Chromatography. a·A Ljunglöf, J Thömmes
Sep 16, 1999·Journal of Chromatography. a·D P RichardsN J Dovichi
Dec 23, 1999·Journal of Colloid and Interface Science·M MalmstenA Ljunglöf
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·B Lebreton, A Lyddiatt
Mar 22, 2002·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·A Adenier, J J Aaron
Jun 13, 2002·Journal of Chromatography. a·Mina LarssonKarl-Gustav Knuuttila
Sep 27, 2002·Biotechnology and Bioengineering·Jürgen HubbuchMaria-Regina Kula
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander V StoyanovSergey N Krylov
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·S R DziennikA M Lenhoff
Jan 16, 2003·Bioconjugate Chemistry·Volker BuschmannMarkus Sauer
Apr 18, 2003·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Susan BingamanRolando E Rumbaut
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Volker KascheMoustafa Gad
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Devanand PintoNorman J Dovichi
Nov 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Judith E BerlierRichard P Haugland
Feb 22, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anuradha Subramanian, Jennifer Hommerding
May 13, 2005·Biotechnology and Bioengineering·S R DziennikA M Lenhoff
Jul 26, 2005·Journal of Chromatography. a·Cristina MartinGiorgio Carta
Oct 15, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sören DooseMarkus Sauer
Nov 18, 2005·Journal of Colloid and Interface Science·E H C Bromley, I Hopkinson
Feb 14, 2006·The Journal of Physical Chemistry. B·Magnus SchröderJürgen Hubbuch
Jun 6, 2006·Biotechnology and Bioengineering·Christopher A TeskeJürgen J Hubbuch
Jul 4, 2006·Biotechnology and Bioengineering·Sun Chau SiuNigel J Titchener-Hooker
Jul 21, 2006·The Journal of Physical Chemistry. B·Christopher A TeskeJürgen Hubbuch
Aug 10, 2006·Biotechnology and Bioengineering·C HarinarayanR van Reis

❮ Previous
Next ❯

Citations

Mar 21, 2008·Bioprocess and Biosystems Engineering·Dirk Weuster-Botz
Oct 4, 2011·Journal of Chromatography. a·Jeremy D KimmelWilliam J Federspiel
Aug 16, 2011·Journal of Biotechnology·Juan M BolivarFernando López-Gallego
Nov 1, 2012·Journal of Separation Science·Rainer Hahn
Jun 25, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Doerte Luensmann, Lyndon Jones
Nov 12, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jeremy D KimmelWilliam J Federspiel
Apr 27, 2018·Physical Chemistry Chemical Physics : PCCP·Alexandra LoukaClaudio Luchinat
Feb 1, 2021·Analytica Chimica Acta·Thomas ThemelisSebastiaan Eeltink
Jul 19, 2012·Analytical Chemistry·Yuewu XiaoMarta Portoles

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.