Confocal microscopy and microbial viability detection for food research

Journal of Food Protection
K Takeuchi, J F Frank

Abstract

Confocal microscopy offers several advantages over other conventional microscopic techniques as a tool for studying the interaction of bacteria with food and the role of food microstructure in product quality and safety. When using confocal microscopy, samples can be observed without extensive preparation processes, which allows for the evaluation of food without introducing artifacts. In addition, observations can be made in three dimensions without physically sectioning the specimen. The confocal microscope can be used to follow changes over a period of time, such as the development of the food structure or changes in microbial population during a process. Microbial attachment to and detachment from food and food contact surfaces with complex three-dimensional (3-D) structures can be observed in situ. The fate of microbial populations in food system depends on processing, distribution, and storage conditions as well as decontamination procedures that are applied to inactivate and remove them. The ability to determine the physiological status of microorganisms without disrupting their physical relationship with a food system can be useful for determining the means by which microorganisms survive decontamination treatments. Con...Continue Reading

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Citations

Jun 15, 2014·Applied and Environmental Microbiology·Isabelle FleurotAgnès Delacroix-Buchet
Jun 6, 2003·Applied and Environmental Microbiology·Danilo ErcoliniChristine E R Dodd
Jan 20, 2018·Parasitology Research·Shahira A Ahmed, Panagiotis Karanis
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Fabián González-RivasJosé Juan Rodríguez-Jerez
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Shuai ZhuangYongkang Luo
Mar 31, 2020·International Journal of Food Microbiology·Raffaella Di CagnoMarco Gobbetti

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