Confocal-Raman Microscopy Characterization of Supported Phospholipid Bilayers Deposited on the Interior Surfaces of Chromatographic Silica

Journal of the American Chemical Society
David A BryceJoel M Harris

Abstract

A common approach to exploring the structure and dynamics of biological membranes is through the deposition of model lipid bilayers on planar supports by Langmuir-trough or vesicle-fusion methods. Planar-supported lipid bilayers have been shown to exhibit structure and properties similar to those of lipid-vesicle membranes and are suitable for biosensing applications. Investigations using these planar-membrane models are limited to high-sensitivity methods capable of detecting a small population of molecules at the interface between a planar support and aqueous solution. In this work, we present evidence that supported-lipid bilayers can be deposited by vesicle fusion onto the interior surfaces throughout the wide-pore network of chromatographic silica particles. The thickness of a 1,2-dimyristoyl- sn-glycero-3-phosphocholine (DMPC) film and headgroup spacing are consistent with a single bilayer of DMPC deposited onto the pore surfaces. The high specific surface area of these materials generates phospholipid concentrations easily detected by confocal-Raman microscopy within an individual particle, which allows the structure of these supported bilayers to be investigated. Raman spectra of porous-silica-supported DMPC bilayers ar...Continue Reading

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Citations

May 23, 2021·Colloids and Surfaces. B, Biointerfaces·Patrick TaiDavid W Everett
Jun 18, 2021·Nature Protocols·Guillermo Bordanaba-FloritJuan M Falcón-Pérez
Mar 28, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·David A BryceJoel M Harris
Jun 7, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Maryam ZareJoel M Harris
Jun 13, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Katarzyna PielaIstván Furó
Nov 14, 2018·The Journal of Physical Chemistry Letters·Vesna ŽivanovićJanina Kneipp
Dec 10, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·ZhangFei SuJacek Lipkowski
Aug 15, 2019·ACS Applied Materials & Interfaces·Ian R BruzasLaura B Sagle
Dec 4, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·David A BryceJoel M Harris

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