Confocal Raman microspectroscopic study of folate receptor-targeted delivery of 6-mercaptopurine-embedded gold nanoparticles in a single cell

Journal of Biomedical Materials Research. Part a
Jin ParkSang-Woo Joo

Abstract

We investigate the cellular uptake behaviors and efficacy of folate-coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate-conjugated AuNPs embedded with a purine analogue cancer drug of 6-mercaptopurine (6MP) were assembled via a 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4-aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy. The internalization of the conjugated AuNPs inside the folate receptor-positive HeLa and KB cells was checked by TEM and dark-field microscopy (DFM) combined with label-free confocal spectroscopy over the depth variable z at a micrometer resolution. DFM live cell imaging of folate-conjugated AuNPs in HeLa cells indicated that the targeted AuNPs appeared to attach on the cell surfaces and enter into the cell with an hour. The cell viability was also compared to estimate the efficacy of folate-conjugated AuNP delivery systems. Folate receptor-targeted AuNP systems appeared to decrease cancer cell viability both in vitro and in v...Continue Reading

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Citations

Jul 13, 2013·Analytical and Bioanalytical Chemistry·Seydou YaoCyril Petibois
Nov 28, 2013·Chemical Reviews·Lev A Dykman, Nikolai G Khlebtsov
May 28, 2019·Chemistry : a European Journal·Nicolas G ChablozJames D E T Wilton-Ely
Jul 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Behdokht BahramiMohammad Hojjat-Farsangi

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