Confocal scanning laser microscope images of hippocampal neurons intracellularly labeled with biocytin

Journal of Neuroscience Methods
J S DeitchJ N Turner

Abstract

We have assessed the properties and usefulness of confocal scanning laser microscopy in the reflection mode for the study of neuronal morphology. In this mode, the confocal microscope detects the light reflected off the specimen as opposed to the light emitted by a fluorescent label. Neurons in slices of rat hippocampus were filled with biocytin and reacted sequentially with avidin-horseradish peroxidase and nickel-intensified diaminobenzidine (DAB/Ni). In all parts of the neuron the DAB/Ni reaction product produced a strong reflection signal in the confocal microscope. The stereo images revealed aspects of three-dimensional hippocampal cell morphology such as the conical shape of the dendritic fields and a characteristic branching pattern of the axon. Labelling neurons intracellularly is an established technique for identifying physiologically-characterized neurons. Recently, confocal microscopy has become a powerful method for examining the three-dimensional morphology of biological specimens. The resulting images in this paper show that reflection-mode confocal microscopy provides an excellent representation of the filled neurons in three dimensions and presents an opportunity for correlative electrophysiological and morphol...Continue Reading

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Citations

Oct 21, 1996·Journal of Neuroscience Methods·L P TolbertJ G Hildebrand
Feb 25, 2003·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Karl-Jürgen Halbhuber, Karsten König
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Jan 1, 1993·Microscopy Research and Technique·D D KunkelP A Schwartzkroin
Apr 1, 1993·Microscopy Research and Technique·B WillisT J Holmes
Apr 4, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·X J SunI A Meinertzhagen
Jun 21, 2006·Aesthetic Plastic Surgery·Hiko Hyakusoku, Takafumi Chin
Jun 27, 2019·Journal of Microscopy·Mayandi SivaguruMakoto Inoue

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