PMID: 11606214Oct 19, 2001Paper

Conformation and other biophysical properties of cyclic antimicrobial peptides in aqueous solutions

The Journal of Peptide Research : Official Journal of the American Peptide Society
M Jelokhani-NiarakiRobert S Hodges

Abstract

As a step towards understanding the mechanism of the biological activity of cyclic antimicrobial peptides, the biophysical properties and conformations of four membrane-active cyclic peptide antibiotics, based on gramicidin S (GS), were examined in aqueous environments. These cyclic peptides, GS10 [cyclo(VKLdYP)2], GS12 [cyclo(VKLKdYPKVKLdYP)], GS14 [cyclo(VKLKVdYPLKVKLdYP)] and [d-Lys]4GS14 [cyclo(VKLdKVdYPLKVKLdYP)] (d-amino acid residues are denoted by d and are underlined) had different ring sizes of 10, 12 and 14 residues, were different in structure and amphipathicity, and covered a broad spectrum of hemolytic and antimicrobial activities. GS10, GS12 and [d-Lys]4GS14 were shown to be monomeric in buffer systems with ionic strength biological environments. GS14 was also monomeric at low concentrations, but aggregated at concentrations > 50 microm. The affinity of peptides for self-assembly and interaction with hydrophobic surfaces was related to their free energy of intermolecular interaction. The effects of variations in salt and organic solvent (trifluoroethanol) concentration and temperature on peptide conformation were also examined. Similar to GS, GS10 proved to have a stable and rather rigid conformation in different...Continue Reading

References

Jul 25, 1976·Journal of Molecular Biology·C Chothia
Mar 27, 1975·Nature·C Chothia
Aug 28, 1990·European Journal of Biochemistry·H Kleinkauf, H von Döhren
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·J A Schellman
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A SternL C Craig
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Feb 1, 1996·Protein Science : a Publication of the Protein Society·T Y Lin, S N Timasheff
Oct 11, 1996·The Journal of Biological Chemistry·L H KondejewskiR S Hodges
Oct 1, 1996·Biophysical Journal·R L Baldwin
Aug 1, 1997·Quarterly Reviews of Biophysics·M G CacaceJ J Ramsden
Apr 18, 1998·Nature Structural Biology·A C GibbsD S Wishart
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Mar 11, 2000·The New England Journal of Medicine·M G Betjes
Feb 1, 1949·Journal of the American Chemical Society·H K SCHACHMAN, M A LAUFFER

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Citations

May 18, 2004·Journal of the American Society for Mass Spectrometry·Brandon T RuotoloDavid H Russell
Mar 13, 2009·Journal of Medicinal Chemistry·Masoud Jelokhani-NiarakiRobert S Hodges
Nov 16, 2011·Biochemistry·Sarah R Dennison, David A Phoenix
Mar 22, 2005·Chirality·Alison A EdwardsGeorge E Tranter
May 13, 2011·Bioorganic & Medicinal Chemistry·Annemiek D KnijnenburgMark Overhand
Aug 31, 2007·Bioorganic & Medicinal Chemistry·Michael A MarquesClay C Wang
Oct 19, 2010·Bioorganic & Medicinal Chemistry·Annemiek D KnijnenburgMark Overhand
Jul 16, 2008·Biophysical Journal·Masoud Jelokhani-NiarakiLaura Wheaton
May 29, 2003·Biophysical Journal·Shelia J ManessR Brian Dyer
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Sergii AfoninAnne S Ulrich
Mar 19, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Dennis Wilkens JuhlBurkhard Bechinger
Jun 26, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·M Jelokhani-NiarakiR S Hodges
Feb 11, 2010·The Journal of Physical Chemistry. B·Adil Khalfa, Mounir Tarek
Jun 29, 2021·Journal of Biomaterials Applications·Lisheng ZhuGang Peng
Jan 4, 2006·The Journal of Organic Chemistry·Parvesh WadhwaniAnne S Ulrich

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