Conformation change in a self-recognizing autotransporter modulates bacterial cell-cell interaction.

The Journal of Biological Chemistry
Victoria GirardMichael Mourez

Abstract

Bacteria mostly live as multicellular communities, although they are unicellular organisms, yet the mechanisms that tie individual bacteria together are often poorly understood. The adhesin involved in diffuse adherence (AIDA-I) is an adhesin of diarrheagenic Escherichia coli strains. AIDA-I also mediates bacterial auto-aggregation and biofilm formation and thus could be important for the organization of communities of pathogens. Using purified protein and whole bacteria, we provide direct evidence that AIDA-I promotes auto-aggregation by interacting with itself. Using various biophysical and biochemical techniques, we observed a conformational change in the protein during AIDA-AIDA interactions, strengthening the notion that this is a highly specific interaction. The self-association of AIDA-I is of high affinity but can be modulated by sodium chloride. We observe that a bile salt, sodium deoxycholate, also prevents AIDA-I oligomerization and bacterial auto-aggregation. Thus, we propose that AIDA-I, and most likely other similar autotransporters such as antigen 43 (Ag43) and TibA, organize bacterial communities of pathogens through a self-recognition mechanism that is sensitive to the environment. This could permit bacteria to...Continue Reading

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Citations

Apr 10, 2013·Research in Microbiology·Jan GrijpstraJan Tommassen
Feb 24, 2011·Infection and Immunity·Jean-Philippe Côté, Michael Mourez
Jan 11, 2011·Journal of Bacteriology·Shirley M EspertGeorge P Munson
Jul 3, 2015·Molecular Microbiology·Zachary C RuheChristopher S Hayes
Sep 9, 2015·Frontiers in Microbiology·Efstathios GiaourisManuel Simões
Nov 26, 2013·Biochimica Et Biophysica Acta·Peter van UlsenJoen Luirink
Mar 4, 2014·Current Opinion in Microbiology·Laetitia Travier, Marc Lecuit
Mar 16, 2017·Frontiers in Microbiology·Jennifer L Hoeflinger, Michael J Miller
Feb 28, 2019·ACS Synthetic Biology·Recep Erdem AhanUrartu Özgür Şafak Şeker

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