Conformation-Dependent Manipulation of Human Islet Amyloid Polypeptide Fibrillation by Shiitake-Derived Lentinan

ACS Applied Materials & Interfaces
Yanru XinFanling Meng

Abstract

Misfolding and aggregation of human islet amyloid polypeptide (hIAPP) into fibrils are important contributions to the pathology of type 2 diabetes. Developing effective inhibitors of protein aggregation and fibrillation has been considered a promising therapeutic approach to preventing and treating type 2 diabetes. Herein, we report that Shiitake-derived polysaccharide lentinan manipulates in vitro hIAPP fibrillation and modulates IAPP-induced cytotoxicity in a conformation-dependent manner. In its triple-helical conformation, lentinan effectively inhibits hIAPP fibrillation, either in bulk solution or in the presence of lipid membrane, suppresses reactive oxygen species (ROS) generation, and attenuates hIAPP-induced cell toxicity. In contrast, lentinan accelerates hIAPP aggregation when it exists in a random-coil conformation and shows no suppression on hIAPP-mediated ROS production. Further investigation shows that the interaction between triple-helical lentinan and monomeric hIAPP is more favorable than the intermolecular binding of hIAPP, which redirects hIAPP aggregates to discrete nontoxic nanocomposites. To the best of our knowledge, this is the first time to report a conformation-dependent inhibition of hIAPP aggregatio...Continue Reading

References

Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkK H Johnson
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G J CooperK B Reid
Nov 14, 1986·Biochemical and Biophysical Research Communications·P WestermarkK Sletten
Apr 3, 2003·Experimental Gerontology·Lucy MarzbanC Bruce Verchere
Mar 6, 2004·Upsala Journal of Medical Sciences·Gunilla T WestermarkArne Andersson
Aug 5, 2004·The Journal of Clinical Endocrinology and Metabolism·Rebecca L HullSteven E Kahn
Sep 24, 2005·The FEBS Journal·Barbara KonarkowskaGarth J S Cooper
Oct 27, 2005·Current Alzheimer Research·Rudy J CastellaniMark A Smith
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Li-Mei YanAphrodite Kapurniotu
Aug 29, 2007·Journal of the American Chemical Society·Andisheh AbediniDaniel P Raleigh
Jul 30, 2008·The Journal of Physical Chemistry. B·Xiaohua WangLina Zhang
Aug 30, 2008·The New England Journal of Medicine·Gunilla T WestermarkUNKNOWN Nordic Network for Clinical Islet Transplantation
Sep 30, 2008·The Journal of Biological Chemistry·Ramona BravoXavier Fernàndez-Busquets
Dec 19, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·C Cabaleiro-LagoS Linse
Jun 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan José Valle-DelgadoXavier Fernàndez-Busquets
Sep 30, 2010·Journal of the American Chemical Society·Fanling MengAndisheh Abedini
Feb 26, 2011·Current Protein & Peptide Science·Dulce Papy-GarciaRita Raisman-Vozari
Jul 12, 2011·Physiological Reviews·Per WestermarkGunilla T Westermark
Aug 15, 2012·Journal of the American Chemical Society·Maarten F M EngelMischa Bonn
Nov 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ali Reza A LadiwalaPeter M Tessier
Apr 13, 2013·Chemical Communications : Chem Comm·Sunil Kumar, Andrew D Miranker
Nov 19, 2013·Critical Reviews in Food Science and Nutrition·Xiaofei XuXuewu Zhang
Mar 22, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tuane C R G VieiraJerson L Silva
Dec 24, 2014·Chemical Communications : Chem Comm·De-Sheng ZhaoYan-Mei Li
Apr 30, 2015·The Journal of Biological Chemistry·Marie E OskarssonGunilla T Westermark
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Pietro SormanniMichele Vendruscolo

❮ Previous
Next ❯

Citations

Mar 30, 2021·ACS Chemical Neuroscience·Yijing TangJie Zheng
Dec 14, 2018·The Journal of Physical Chemistry Letters·Qunxing HuangYanlian Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.