PMID: 12015423May 17, 2002Paper

Conformation-dependent regulation of inward rectifier chloride channel gating by extracellular protons

The Journal of Physiology
Jorge ArreolaJames E Melvin

Abstract

We have investigated the gating properties of the inward rectifier chloride channel (Cl(ir)) from mouse parotid acinar cells by external protons (H(+)(o)) using the whole-cell patch-clamp technique. Increasing the pH(o) from 7.4 to 8.0 decreased the magnitude of Cl(ir) current by shifting the open probability to more negative membrane potentials with little modification of the activation kinetics. The action of elevated pH was independent of the conformational state of the channel. The effects of low pH on Cl(ir) channels were dependent upon the conformational state of the channel. That is, application of pH 5.5 to closed channels essentially prevented channel opening. In contrast, application of pH 5.5 to open channels actually increased the current. These results are consistent with the existence of two independent protonatable sites: (1) a site with a pK near 7.3, the titration of which shifts the voltage dependence of channel gating; and (2) a site with pK = 6.0. External H(+) binds to this latter site (with a stoichiometry of two) only when the channels are closed and prevent channel opening. Finally, block of channels by Zn(2+) and Cd(2+) was inhibited by low pH media. We propose that mouse parotid Cl(ir) current has a bi...Continue Reading

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Citations

Apr 23, 2010·The Journal of Membrane Biology·Akihiro InagakiToru Ishikawa
Mar 23, 2004·Biochemical and Biophysical Research Communications·Patricia Perez-Cornejo, Jorge Arreola
Jul 20, 2012·Chemical Reviews·Ivana Y Kuo, Barbara E Ehrlich
Nov 16, 2005·The Journal of General Physiology·Jose Antonio de SantiagoJorge Arreola
Sep 2, 2010·The Journal of General Physiology·Antonella GradognaMichael Pusch
Feb 4, 2003·The Journal of Physiology·Jorge Arreola, James E Melvin
Feb 16, 2005·Annual Review of Physiology·Thomas J JentschAnselm A Zdebik
Nov 19, 2013·Journal of Molecular and Cellular Cardiology·Yosuke OkamotoKyoichi Ono
Sep 18, 2013·Pflügers Archiv : European journal of physiology·Eloy G Moreno-GalindoRicardo A Navarro-Polanco
Nov 18, 2003·The Journal of Physiology·María Isabel NiemeyerFrancisco V Sepúlveda
Apr 1, 2003·The Journal of Physiology·H Criss Hartzell, Zhiqiang Qu
Jan 16, 2004·The Journal of Physiology·Leandro ZúñigaFrancisco V Sepúlveda
Sep 20, 2008·The Journal of Physiology·Jennie Garcia-OlivaresChristoph Fahlke
May 26, 2010·The Journal of Physiology·Jorge E Sánchez-RodríguezJorge Arreola
Dec 17, 2015·The Journal of General Physiology·José J De Jesús-PérezJorge Arreola
Mar 17, 2010·Biochemical and Biophysical Research Communications·Kyunghee X Kim, Daniel C Marcus
Mar 13, 2015·The Journal of Physiology·Alejandro Barrallo-GimenoRaúl Estévez
Apr 9, 2005·IEEE Transactions on Nanobioscience·Michael Pusch, Thomas J Jentsch
Jul 5, 2013·Biophysical Journal·Antonella Gradogna, Michael Pusch
Nov 20, 2016·The Journal of Physiology·Silvia Cruz-RangelJorge Arreola
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
May 31, 2006·Reproduction, Nutrition, Development·Céline AuzanneauClarisse Vandebrouck
Nov 21, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Khalid AbdounHolger Martens
Apr 27, 2002·The Journal of Biological Chemistry·Keith NehrkeJames E Melvin
Apr 2, 2009·The Journal of Physiology·Michael Pusch, Giovanni Zifarelli
May 11, 2005·The Journal of Biological Chemistry·Fiona C BrittonDayue Duan
Jul 3, 2007·The Journal of Experimental Biology·Uwe RoseChristian Walther
Jun 25, 2021·Scientific Reports·José J De Jesús-PérezJorge Arreola

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