PMID: 6269Apr 15, 1976

Conformation of Escherichia coli glutamic acid tRNA II as studied by hydrogen-tritium exchange catalyzed by cysteine methyl ester

European Journal of Biochemistry

Abstract

Incubation of CMP in 2H2O with 0.5M cysteine methyl ester at p2H 5 and 37 degrees C for 24 h resulted in 43% exchange of 5-H to 5-2H. No deamination of the cytosine nucleus was noted during this treatment. Native and denatured DNA samples from calf thymus were treated in 3H2O with cysteine methyl ester at pH 5 and 37 degrees C for 24 h and incorporation of tritium into each DNA base was determined by enzymic digestion of the treated DNA. The order of the specific radioactivity found was cytosine greater than guanine greater than adenine greater than thymine for denatured DNA and guanine greater than adenine approximately cytosine greater than thymine for native DNA. The ratio of radioactivity for denatured/native was 11.6 for cytosine, 1.5 for guanine, 1.8 for adenine and 1.1 for thymine. Hence the incorporation in cytosine under the reaction conditions is preferential for single-stranded, nonhelical regions of DNA. Escherichia coli glutamic acid tRNA II was treated in 3H2O with 1.24 M cysteine methyl ester at pH 5 and 37 degrees C. The 24-h-treated tRNA was digested with ribonuclease T1 and the fragments were fractionated. Each fragment was then digested with ribonuclease T2 into mononucleotides and the radioactivity distribut...Continue Reading

References

Jan 1, 1966·Progress in Nucleic Acid Research and Molecular Biology·R W Chambers
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R G ShulmanD R Kearns
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R C Gamble, P R Schimmel
Aug 16, 1974·Nature·J D RobertusA Klug
Mar 1, 1974·FEBS Letters·S IidaH Hayatsu
Dec 13, 1972·Journal of the American Chemical Society·Y WatayaY Kawazoe
Apr 1, 1973·Journal of Biochemistry·Y WatayaY Kawazoe
Jul 11, 1973·Journal of the American Chemical Society·M SonoH Hayatsu
Jul 28, 1971·Journal of Molecular Biology·A R CashmoreJ D Smith
Apr 21, 1971·Nature: New Biology·T Cashmore
Aug 11, 1971·Nature: New Biology·Z KućanR W Chambers
Sep 1, 1965·Journal of Molecular Biology·F SangerB G Barrell
Feb 11, 1970·Journal of the American Chemical Society·H HayatsuK Kazushige
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Related Concepts

Cysteine Hydrochloride
Deuterons
DNA, Double-Stranded
Alkalescens-Dispar Group
Glutamic Acids
Hydrogen-Ion Concentration
RNA Conformation
RNA Denaturation
RNA, Bacterial
Transfer RNA

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