Conformation of human leucocyte antigen-C molecules at the surface of human trophoblast cells.

Immunology
Richard AppsAshley Moffett

Abstract

Human leucocyte antigen (HLA)-C is expressed at lower levels than other classical HLA-I molecules on somatic cells. Surface HLA-C proteins can occur as conventionally beta(2)-microglobulin (beta2m)-associated complexes or as open conformers dissociated from peptide and/or beta(2)m. We investigated the conformation of HLA-C molecules on normal human trophoblast cells, which invade the maternal decidua during placentation. A panel of monoclonal antibodies to different conformations of HLA-I molecules was used in flow cytometry and surface immunoprecipitation experiments. On the surface of trophoblast cells only beta(2)m-associated complexes of HLA-C molecules were detected. In contrast, both open conformers and beta(2)m-associated HLA-C could be detected on other cells from the decidua, HLA-C-transfectants and cell lines. The levels of HLA-C expressed on primary trophoblast cells could be detected by antibodies specific to non-beta(2)m-associated conformations because binding was seen after acid-induced denaturation of surface proteins. In contrast to HLA-G molecules on trophoblasts, we found no evidence for the presence of disulphide-linked multimers of HLA-C complexes. These results show that most HLA-C molecules present at the...Continue Reading

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Citations

Oct 26, 2010·The Journal of Clinical Investigation·Susan E HibyAshley Moffett
Jul 22, 2014·Cellular & Molecular Immunology·Kenneth D BeamanAlice Gilman-Sachs
Oct 27, 2015·Journal of Reproductive Immunology·A van EgmondF H J Claas
Nov 22, 2015·American Journal of Reproductive Immunology : AJRI·Svetlana V DambaevaKenneth D Beaman
Jun 7, 2011·Journal of Reproductive Immunology·Astrid Hammer
Apr 8, 2009·The American Journal of Pathology·Samantha D SmithRebecca L Jones
Jul 29, 2008·Seminars in Immunology·John Trowsdale, Ashley Moffett
Mar 2, 2011·Immunology·Marie-Eve BlaisSarah Rowland-Jones
Aug 19, 2015·Immunological Reviews·Ashley Moffett, Francesco Colucci
Aug 30, 2011·Journal of Leukocyte Biology·Olympe ChazaraAshley Moffett
Jan 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Des C JonesRachel L Allen
May 24, 2018·Annals of Hematology·Elsa M CardosoFernando A Arosa
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew M SharkeyAshley Moffett
Nov 30, 2018·Frontiers in Immunology·Jürgen PollheimerMartin Knöfler
Jul 13, 2012·The Journal of Reproduction and Development·Lei ShuKechun Zhang
Feb 3, 2021·Human Reproduction Update·Indra Díaz-HernándezFrancisco Domínguez

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