PMID: 11321678Apr 26, 2001Paper

Conformation of NADH studied by fluorescence excitation transfer spectroscopy

Biophysical Chemistry
R V HullR J Hoobler

Abstract

Dihydronicotinamide adenine dinucleotide (NADH), is known to stack in two limiting conformations. Surprisingly, previous experimental work on NADH has not clearly defined whether this folding and unfolding process can be described as first order (involving only two states) or whether one or more intermediates must be included in the description. In addition, a large disparity exists between reported equilibrium constants for the aqueous solution at room temperature. Using methanol as a denaturant, we have used fluorescence excitation transfer spectroscopy to probe the stacking/un-stacking equilibrium. Our results can be represented using a simple two state model. Furthermore, the mole fraction of aqueous NADH in the stacked configuration is significantly higher than previously reported. Using a thermodynamic two state model, we have determined deltaG(o)(H2O) = 8.8 +/- 1.4 kJ mol(-1). From excitation energy transfer measurements the fraction of NADH in the folded form at 295 K was determined to be 0.55.

References

Sep 6, 1967·Biochimica Et Biophysica Acta·S FreedL A Tumerman

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Citations

Feb 25, 2011·The Journal of Biological Chemistry·Chuanwu XiaJung-Ja P Kim
Jun 22, 2011·The Journal of Biological Chemistry·Federico I RosellA Grant Mauk
Sep 9, 2015·Journal of Inorganic Biochemistry·Elena FormosoXabier Lopez
Sep 7, 2006·The Journal of Applied Psychology·Kathryne E DupréColette Hoption
Jan 17, 2019·Chemistry : a European Journal·Jonas FeldmannYitzhak Tor
Aug 9, 2020·Physical Chemistry Chemical Physics : PCCP·Ioanna A GorbunovaOleg S Vasyutinskii
Jan 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simin CaoLudwig Brand
Jan 17, 2020·The Journal of Physical Chemistry. B·Simin CaoJay R Knutson
Jun 25, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dylan PaloPaul Urayama
Jul 15, 2020·The Journal of Physical Chemistry. B·Simin CaoLudwig Brand
Dec 5, 2009·The Journal of Physical Chemistry. a·Limor RadoszkowiczMenachem Gutman
Jul 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dina M BeltukovaOleg S Vasyutinskii
Oct 19, 2017·Journal of the American Chemical Society·Alexander R RoviraYitzhak Tor
Apr 26, 2019·The Journal of Physical Chemistry. B·Thomas S BlackerAngus J Bain
Jul 22, 2017·The Journal of Physical Chemistry. B·Zsuzsanna HeinerGéza I Groma
Nov 12, 2020·The Journal of Physical Chemistry. B·Ioanna A GorbunovaOleg S Vasyutinskii
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin HeidelmanPaul Urayama

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