PMID: 7213649Dec 16, 1980Paper

Conformation of pardaxin, the toxin of the flatfish Pardachirus marmoratus

Biochimica Et Biophysica Acta
N Primor, A T Tu

Abstract

Pardaxin is the toxic component isolated from the secretion of the Red Sea flatfish Pardachirus marmoratus. Pardaxin has attracted considerable attention recently because of its use as shark repellent. Conformational information regarding the peptide backbone, disulfide bonds, and tyrosine chromophores was obtained using Raman and circular dichroism (CD) spectroscopy. Analysis of the Raman spectra indicates that the toxin consists of 39% alpha-helix, 23% beta-structure, and 39% random coil. The occurrence of a band at 510--512 cm-1 indicates the conformational geometry of the disulfide C-C-S-S-C-C linkages to be gauche-gauche-gauche. From the lack of a peak in the 2500--2700 cm-1 region we concluded that all half-cystines are involved in disulfide linkages. The far-ultraviolet CD spectrum of pardaxin showed positive 196 and negative 208 and 222 nm bands. The best fit secondary structure based on the CD ellipticities was found to be: 23% alpha-helix, 21% beta-structure, and 56% random coil. Under highly acidic and alkaline conditions the far-ultraviolet pardaxin spectra showed extensive, reversible loss of its helical structure. Due to certain biological characteristics of pardaxin the possible influence of salt on pardaxin's co...Continue Reading

References

Oct 27, 1976·Journal of the American Chemical Society·J L LippertP J Desmeules
Jan 1, 1976·Toxicon : Official Journal of the International Society on Toxinology·J Parness, E Zlotkin
May 1, 1973·Archives of Biochemistry and Biophysics·N T YuD C O'Shea
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J Bandekar, S Krimm

Citations

Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·N Primor, P Lazarovici
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·S D AirdA L Bieber
Jan 1, 1984·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·N PrimorJ N Forrest
Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Jul 21, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ammad Ahmad FarooqiHsueh-Wei Chang
Oct 13, 2016·Developmental and Comparative Immunology·Abirami ArasuJesu Arockiaraj

Related Concepts

Pardachirus toxin
Metazoa
Circular Dichroism, Vibrational
Fish Venoms
Hydrogen-Ion Concentration
Protein Conformation
Sodium Chloride, (24)NaCl
Solutions
Raman Optical Activity Spectroscopy

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