Conformation of the oligosaccharide chain of G(M1) ganglioside in a carbohydrate-enriched surface

Biophysical Journal
P BroccaSandro Sonnino

Abstract

The solution structure of ganglioside G(M1) carbohydrate moiety at the surface of a 102-kDa lipid-modified-G(M1) micelle is investigated by high-resolution 1H-NMR in H2O. The micellar surface can be considered a cluster-like lateral distribution of the gangliosides, each single monomer being anchored in a carbohydrate-enriched model membrane matrix. 1H NOESY measurements at short mixing times reveal a rigid trisaccharide core -beta-GalNAc-(1-4)-[alpha-Neu5Ac-(2-3)]-beta-Gal- and a more flexible beta-Gal-(1-3)-beta-GalNAc- terminal glycosidic bond. In the lipid-modified G(M1) ganglioside micellar system, there is no evidence that intermolecular side-by-side carbohydrate interactions modulate, or alter in any way, the head-group spatial arrangement. Possible intermonomer interactions at the level of the branched trisaccharide portion were further investigated on mixed micelles of natural N-glycolyl- and N-acetylneuraminic acid containing G(M1) in D2O, taking advantage of the different NMR features of N-glycolyl- and N-acetylneuraminic acids, which allow discrimination between sialic acid ring proton signals. Measurements of the water/ganglioside-OH proton chemical exchange rates suggest hydroxyl group involvement at position 8 of...Continue Reading

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Citations

Jul 25, 2003·The Journal of Membrane Biology·J StrancarS Pecar
Feb 4, 2010·Molecular Neurobiology·Marco PiccininiAlessandro Prinetti
Oct 12, 2004·Journal of Biomolecular Structure & Dynamics·D Jeya Sundara Sharmila, K Veluraja
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Jan 15, 2008·Biophysical Journal·Shelli L FreyKa Yee C Lee
May 2, 2015·Glycoconjugate Journal·Maho Yagi-Utsumi, Koichi Kato
Oct 13, 2010·Biochimica Et Biophysica Acta·Anindita GayenChaitali Mukhopadhyay
Mar 13, 2015·Molecular Neurobiology·Massimo AureliSandro Sonnino
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Sep 20, 2006·Glycobiology·Sandro SonninoAlessandro Prinetti
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May 25, 2002·Journal of Biomolecular Structure & Dynamics·Debjani Roy, Chaitali Mukhopadhyay
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Jun 28, 2020·FEBS Letters·Maria J SarmentoRadek Šachl
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May 19, 2021·FEBS Open Bio·Giulia LunghiSandro Sonnino
Aug 10, 2019·The Journal of Physical Chemistry. B·Michael C OwenRobert Vácha
Oct 7, 2008·Biochimica Et Biophysica Acta·Alessandro PrinettiSandro Sonnino

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