Conformational activation of visual rhodopsin in native disc membranes.

Science Signaling
Erik MalmerbergRichard Neutze

Abstract

Rhodopsin is the G protein-coupled receptor (GPCR) that serves as a dim-light receptor for vision in vertebrates. We probed light-induced conformational changes in rhodopsin in its native membrane environment at room temperature using time-resolved wide-angle x-ray scattering. We observed a rapid conformational transition that is consistent with an outward tilt of the cytoplasmic portion of transmembrane helix 6 concomitant with an inward movement of the cytoplasmic portion of transmembrane helix 5. These movements were considerably larger than those reported from the basis of crystal structures of activated rhodopsin, implying that light activation of rhodopsin involves a more extended conformational change than was previously suggested.

References

Feb 1, 1987·Biophysical Journal·K J RothschildW J DeGrip
Jan 1, 1980·Methods in Enzymology·W J De GripS L Bonting
Sep 3, 1998·The Journal of Biological Chemistry·F DeLangeK J Rothschild
Jan 6, 1999·Biophysical Journal·D P TielemanH J Berendsen
Aug 5, 2000·Science·K PalczewskiM Miyano
Jan 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuji OkadaYoshinori Shichida
Jan 10, 2003·Nature·Dimitrios FotiadisKrzysztof Palczewski
Mar 29, 2003·The Journal of Biological Chemistry·Yan LiangAndreas Engel
Jun 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ashish B PatelSteven O Smith
Aug 26, 2004·Journal of Molecular Biology·Tetsuji OkadaVolker Buss
Oct 7, 2004·Journal of Molecular Biology·Paul J R SpoonerAnthony Watts
Oct 20, 2004·Journal of Molecular Biology·Jade LiGebhard F X Schertler
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 29, 2006·Current Opinion in Structural Biology·Dimitrios FotiadisAndreas Engel
Apr 6, 2006·Angewandte Chemie·Hitoshi Nakamichi, Tetsuji Okada
Apr 26, 2006·Biochemistry·Ana Karin KusnetzowWayne L Hubbell
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hitoshi Nakamichi, Tetsuji Okada
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·David SalomKrzysztof Palczewski
Apr 24, 2007·Biophysical Journal·Hitoshi NakamichiTetsuji Okada
Sep 11, 2007·Journal of Molecular Biology·Jörg StandfussGebhard F X Schertler
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christian AltenbachWayne L Hubbell
Jun 20, 2008·Nature·Jung Hee ParkOliver Peter Ernst
Jul 23, 2008·Acta Crystallographica. Section D, Biological Crystallography·Ronald E Stenkamp
Sep 27, 2008·Nature·Patrick ScheererOliver P Ernst
Nov 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mohana MahalingamReiner Vogel
May 1, 2009·Angewandte Chemie·Kristoffer HaldrupMartin M Nielsen
Sep 15, 2009·Structure·Magnus AnderssonRichard Neutze
Sep 18, 2009·The Journal of Physical Chemistry. B·Sena AhnHyotcherl Ihee
Mar 3, 2010·Annual Review of Biophysics·Steven O Smith
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hyun Sun ChoPhilip Anfinrud
Oct 5, 2010·Nature Methods·Sebastian WestenhoffRichard Neutze
Oct 7, 2010·Biophysical Journal·Clint L MakinoTetsuji Okada
Feb 2, 2011·The Journal of Biological Chemistry·Penelope J CrossEmily J Parker
Mar 11, 2011·Nature·Jörg StandfussGebhard F X Schertler
Mar 11, 2011·Nature·Hui-Woog ChoeOliver P Ernst
Jun 2, 2011·Journal of the American Chemical Society·Pradeep L RamachandranJasper J van Thor
Jul 21, 2011·Nature·Søren G F RasmussenBrian K Kobilka

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Citations

Sep 8, 2015·Current Opinion in Structural Biology·Matteo LevantinoArwen R Pearson
Jan 23, 2016·Structural Dynamics·Valérie PanneelsGebhard Schertler
Sep 16, 2016·The Journal of Physical Chemistry Letters·Utsab R ShresthaXiang-Qiang Chu
Oct 11, 2016·The Journal of Physical Chemistry Letters·Suchithranga M D C PereraMichael F Brown
Aug 22, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yasushi ImamotoYoshinori Shichida
Aug 15, 2015·Physical Chemistry Chemical Physics : PCCP·Cheolhee YangHyotcherl Ihee
May 10, 2020·Journal of Synchrotron Radiation·Jungmin KimHyotcherl Ihee
Mar 29, 2020·Science Advances·Harsha RavishankarMagnus Andersson
Apr 19, 2019·Journal of Applied Crystallography·Amit SharmaRichard Neutze
Jan 8, 2021·The Journal of Membrane Biology·Fredrik Orädd, Magnus Andersson
Nov 30, 2018·The Journal of Physical Chemistry Letters·Suchithranga M D C PereraMichael F Brown
Oct 4, 2019·The Journal of Physical Chemistry. B·Yasushi ImamotoYoshinori Shichida
Oct 5, 2021·Acta Crystallographica. Section D, Structural Biology·Diana C F MonteiroArwen R Pearson
Nov 18, 2021·Science Advances·Fredrik OräddMagnus Andersson

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