PMID: 7543469Aug 1, 1995Paper

Conformational analysis of the Campylobacter jejuni porin

Journal of Bacteriology
J M BollaJ M Pages

Abstract

The major outer membrane protein (MOMP) of Campylobacter jejuni was purified to homogeneity by selective solubilization and fast protein liquid chromatography. The amino acid composition of the MOMP indicates the presence of cysteine residues. The amino-terminal sequence, determined over 31 residues, shows no significant homology with any other porin from gram-negative bacteria except in a discrete region. Immunocross-reactivity between Escherichia coli OmpC and the MOMP was analyzed, and a common antigenic site between these two porins was identified with an anti-peptide antibody. From circular dichroism and immunological investigations, the existence of a stable folded monomer, containing a high level of beta-sheet secondary structure, is evident. Conformational analyses show the presence of a native trimeric state generated by association of the three folded monomers; the stability of this trimer is reduced compared with that of E. coli porins. This study clearly reveals that the C. jejuni MOMP is related to the family of trimeric bacterial porins.

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Citations

Feb 1, 1996·FEMS Microbiology Letters·V SimonetJ M Pages
Jul 5, 2005·Foodborne Pathogens and Disease·Shouxiong HuangQijing Zhang
Jan 1, 1996·Microbiology and Immunology·K AmakoA Takade
Jun 19, 2007·FEMS Microbiology Letters·Malgorzata Krause-GruszczynskaSteffen Backert
Feb 28, 2003·The Journal of Biological Chemistry·Valérie C SimonetAnne H Delcour
Dec 14, 2006·Proteomics·Laurent MamelliJean-Michel Bolla
May 15, 2007·Canadian Journal of Microbiology·Clifford G ClarkLai-King Ng
Feb 15, 2013·Virulence·Nicole M Iovine
Sep 28, 2018·PloS One·Charlotte VanmarsenilleHenri De Greve
Feb 23, 2000·FEMS Immunology and Medical Microbiology·B JanvierJ Louis Fauchère
Jul 3, 2007·Journal of Bacteriology·Guifeng SunLuis M de la Maza
Nov 24, 2005·Antimicrobial Agents and Chemotherapy·Axel SiroyEmmanuelle Dé
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Dec 6, 2020·Journal of Veterinary Pharmacology and Therapeutics·Zhiqun Lei, Aman Karim
Nov 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dedieu LucBolla Jean Michel
Feb 13, 2001·Biochemical and Biophysical Research Communications·G LabesseJ M Bolla
Oct 13, 2001·Biochimica Et Biophysica Acta·C El HamelG Molle
Jan 28, 1999·Research in Microbiology·K KelleJ M Bolla
May 8, 2004·Journal of Clinical Microbiology·Luc DedieuJean-Michel Bolla
Apr 20, 2006·International Journal of Biological Macromolecules·Laurent MamelliJean-Michel Bolla

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Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.