PMID: 9531427Apr 8, 1998Paper

Conformational and topological requirements of cell-permeable peptide function

The Journal of Peptide Research : Official Journal of the American Peptide Society
C DuY Z Lin

Abstract

Cell-permeable peptide import recently was developed to deliver synthetic peptides into living cells for studying intracellular protein functions. This import process is mediated by an N-terminal carrier sequence which is the hydrophobic region of a signal peptide. In this study, the conformational consequence of the interaction of cell-permeable peptides with different mimetic membrane environments was investigated by circular dichroism analysis. We showed that cell-permeable peptides adopted alpha-helical structures in sodium dodecyl sulfate (SDS) micelles or aqueous trifluoroethanol (TFE). The potency of these peptides in forming helical structures is higher in an amphiphilic environment (SDS) than in a hydrophobic environment (TFE), suggesting that some hydrophilic molecules associated with the cell membrane may be involved in peptide import. We also studied topological requirements of cell-permeable peptide function. We demonstrated that peptides containing the carrier sequence in their C-termini can also be imported into cells efficiently. This important discovery can avoid repetitious synthesis of the membrane-translocating sequence for peptides with different functional cargoes and is potentially useful for developing a...Continue Reading

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Citations

Apr 29, 1998·Nature Biotechnology·M RojasY Z Lin
Nov 10, 2001·Annual Review of Phytopathology·S E GoldA D Martínez-Espinoza
Apr 1, 2004·Biopolymers·Jimena Fernández-CarneadoErnest Giralt
Oct 3, 2003·Journal of Molecular Recognition : JMR·T LetohaB Penke
Jun 14, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ana L MoraMauricio Rojas
Sep 21, 2000·European Journal of Biochemistry·A SchellerJ Oehlke
Nov 4, 2000·Expert Opinion on Investigational Drugs·L A Kueltzo, C R Middaugh
Oct 18, 2000·Advanced Drug Delivery Reviews·S Dokka, Y Rojanasakul
Feb 26, 2000·Trends in Pharmacological Sciences·M LindgrenU Langel
Oct 16, 2004·Molecular and Cellular Neurosciences·Gunnar P H Dietz, Mathias Bähr

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