PMID: 2489093Oct 1, 1989Paper

Conformational change and aggregation of concanavalin A at high temperatures

International Journal of Biological Macromolecules
H MaedaH Kanoh

Abstract

Increase of the beta sheet content and aggregation of concanavalin A (con A) induced at about 60 C were followed with circular dichroism (c.d.) and scattered light intensity (I90) on both metal-complexed and demetallized species. The conversion occurred at a higher temperature for metal-complexed species than for demetallized one. A concentration-independent conversion curve of metal-complexed species, obtained for a concentration range below around 6 microgram/ml (6 x 10(-3) kg m-3) with a midpoint at 57 degrees C, was well described in terms of a conformational equilibrium between two conformers. However, aggregation did exist even at a low concentration of 1 microgram/ml. Aggregation also occurred without the conformational change as found at the initial stage or in Tris buffer, which suggested the absence of direct coupling between the conformational change and the aggregation. Changes of c.d. at 222 nm, expected to represent the main chain conformation, differed from those at 290 nm reflecting the environment of side chain chromophores. Time courses of three properties examined, c.d. at 222 nm, at 290 nm, and I90, always exhibited a lag in the case of metal-complexed species while the lag was not observed in the case of de...Continue Reading

References

Jul 1, 1978·FEBS Letters·E I Tiktopulo, P L Privalov
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G N ReekeG M Edelman
Jun 25, 1979·Journal of Molecular Biology·M ShohamA J Kalb
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Jan 1, 1980·European Journal of Biochemistry·C Borrebaeck, B Mattiasson

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Citations

Aug 13, 2005·The Protein Journal·Motonori KudouMasahiro Takagi

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