Conformational change and destabilization of cataract gammaC-crystallin T5P mutant

FEBS Letters
Ling Fu, Jack J-N Liang

Abstract

Human lens gammaC-crystallin and T5P mutant were cloned, and their biophysical properties and thermodynamic stability were studied. CRYGC (T5P) is one of the many gamma-crystallin mutant genes for autosomal dominant congenital cataracts. This mutation is associated with Coppock-like cataract, and has the phenotype of a dust-like opacity of the fetal lens nucleus. During cloning and overexpression, the majority of T5P mutant was found in the inclusion body. This property is unique among the many cataract gamma-crystallin mutant genes. It is thus worthwhile to study what factors contribute to this unique property of gammaC-crystallin. One possibility is changes in conformation and stability, which can be studied using spectroscopic measurements. In this study, conformational change was studied by circular dichroism and fluorescence measurements, and conformational stability was determined by thermal unfolding probed by Trp fluorescence and time-dependent light scattering. The T5P mutation obviously changes conformation and decreases conformational stability.

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Nov 9, 2001·The Journal of Biological Chemistry·Ling Fu, Jack J-N Liang

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Citations

Mar 8, 2011·Cell Stress & Chaperones·Shuhua SongToshimichi Shinohara
Nov 30, 2002·Journal of Structural Biology·Ling Fu, Jack J-N Liang
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Priya R BanerjeeJayanti Pande
Nov 6, 2012·The Journal of Biological Chemistry·Fangling JiAngela M Gronenborn
Jul 14, 2010·European Journal of Medical Genetics·Bingyu Huang, Weigong He
Jul 28, 2005·Protein Science : a Publication of the Protein Society·Shannon L FlaughJonathan King
Aug 24, 2004·Protein Science : a Publication of the Protein Society·Jack J-N Liang
Jun 6, 2006·Protein Science : a Publication of the Protein Society·Jack J Liang, Bing-Fen Liu
Sep 29, 2004·Journal of Molecular Biology·P EvansC Slingsby
Oct 15, 2013·Biochimica Et Biophysica Acta·Kai ZhangYong-Bin Yan
Aug 8, 2006·The Journal of Biological Chemistry·Shannon L FlaughJonathan King
May 27, 2016·Journal of Molecular Recognition : JMR·Mohd Shahnawaz KhanAbdulrahman M Al-Senaidy
Sep 1, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J A Domínguez-CalvaL Quintanar
Nov 15, 2003·The Journal of Biological Chemistry·Sarah MeehanCait E MacPhee

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