Conformational changes and catalytic competency of hydrolases adsorbing on fumed silica nanoparticles: II. Secondary structure

Colloids and Surfaces. B, Biointerfaces
Juan C CruzM E Rezac

Abstract

Secondary conformational analysis via Circular Dichroism (CD) and Amide-I FTIR was applied to preparations of Candida antarctica Lipase B (CALB), subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) on fumed silica to confirm that the "hardness" and packing density of the enzymes on the solid fumed silica nanoparticle surface can be used to rationalize the variable enzyme-dependent changes of catalytic competency with surface coverage. "Soft" enzymes should be immobilized at a surface coverage where enzyme-enzyme interactions predominate thereby preventing detrimental structural changes caused by enzyme-support interactions, while "hard" enzymes can be immobilized at low to intermediate surface coverage with good catalytic performance. Multi-layered coverage reduces the superficial average catalytic performance in all cases due to mass transfer limitations.

References

Jul 1, 1992·Biotechnology Progress·J S Dordick
Apr 8, 1992·Biochimica Et Biophysica Acta·J Bandekar
Jan 15, 1992·European Journal of Biochemistry·M N Gupta
Dec 1, 1994·Journal of Pharmaceutical Sciences·H R CostantinoA M Klibanov
Jan 1, 1993·Progress in Biophysics and Molecular Biology·J L ArrondoF M Goñi
Oct 15, 1996·Archives of Biochemistry and Biophysics·A DongJ F Carpenter
Nov 13, 1998·Journal of Pharmaceutical Sciences·H R CostantinoK Griebenow
Feb 18, 1999·Current Opinion in Chemical Biology·Y L Khmelnitsky, J O Rich
Mar 6, 1999·Current Opinion in Microbiology·J S DordickM V Sergeeva
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·H FabianC P Schultz
Feb 19, 2000·Current Opinion in Chemical Biology·P J Halling
Aug 15, 2000·Angewandte Chemie·G Carrea, S Riva
Dec 2, 2000·Biochimie·C M Carvalho, J M Cabral
Jan 3, 2001·Biotechnology and Bioengineering·J O Rich, Y L Khmelnitsky
Jun 19, 2001·Biochimica Et Biophysica Acta·K ZhuP K Kinnunen
Jun 7, 2002·Applied Biochemistry and Biotechnology·Ai-Xin YanYun-Hua Ye
Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Mattias PerssonPatrick Adlercreutz
Aug 29, 2003·Current Opinion in Biotechnology·Alexander M Klibanov
Oct 7, 2003·Annual Review of Biomedical Engineering·Jennifer L West, Naomi J Halas
Jul 6, 2004·European Journal of Biochemistry·Keith A ObergErik Goormaghtigh
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Alexey A VertegelJonathan S Dordick
Jul 31, 2004·Methods : a Companion to Methods in Enzymology·Heinz Fabian, Dieter Naumann
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Douglas S Clark
Sep 28, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Eugenii Katz, Itamar Willner
Oct 19, 2004·Biochimica Et Biophysica Acta·Arimatti JutilaPaavo K J Kinnunen
Apr 26, 2005·The Analyst·Joseph Wang
Jul 19, 2005·Journal of Colloid and Interface Science·V M Gun'koR Leboda
Jul 20, 2005·Biochimica Et Biophysica Acta·Sharon M KellyNicholas C Price
Mar 23, 2006·Journal of the American Chemical Society·Paul RoachCarole C Perry
Apr 29, 2006·Journal of Colloid and Interface Science·V M Gun'koW Janusz
May 24, 2006·Biochimica Et Biophysica Acta·Ashok GanesanPeter J Halling
Jun 14, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Prashanth AsuriJonathan S Dordick

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Citations

Feb 11, 2016·ACS Nano·Xing MaSamuel Sánchez
Mar 19, 2013·Carbohydrate Polymers·Thayyath Sreenivasan Anirudhan, Sylaja Raveendran Rejeena
Nov 18, 2015·Advanced Materials·Xu-Dong WangChristof M Niemeyer
Jan 25, 2012·Colloids and Surfaces. B, Biointerfaces·Joey N Talbert, Julie M Goddard
May 15, 2015·Colloids and Surfaces. B, Biointerfaces·Zigmas BaleviciusGintaras Valincius
Jul 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bhagwati SharmaTridib K Sarma
Nov 7, 2018·Biomaterials·Arnold LeidnerChristof M Niemeyer

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