PMID: 4287181Jan 1, 1966Paper

Conformational changes and the regulation of glutamate-dehydrogenase activity

The Biochemical Journal
P M Bayley, G K Radda

Abstract

1. The effect of NADH and the non-competitive inhibitor GTP on the optical-rotatory-dispersion properties of glutamate dehydrogenase has been studied. 2. Analysis of the data in terms of the a(0) and b(0) parameters of the Moffitt-Yang equation indicates that a conformational change is induced either by NADH or by GTP in the presence of small amounts of NADH. 3. Sedimentation measurements under comparable conditions showed that the enzyme reversibly dissociates into sub-units but that this dissociation is only secondary to the conformational changes. 4. Fluorescence measurements showed that the binding constant of NADH and the number of binding sites on the enzyme increased in the presence of GTP. 5. This is confirmed by studies of fluorescence polarization, which in addition showed that the movement of NADH on the enzyme surface is more restricted in the presence of GTP. 6. The relation of these results to possible regulatory mechanisms is discussed.

Citations

Nov 21, 1972·European Journal of Biochemistry·J A ShaferE Antonini
Aug 31, 1968·Nature·A D Malcolm, G K Radda
Apr 1, 1982·European Journal of Biochemistry·M Nemat-Gorgani, K Karimian
Jun 9, 1972·European Journal of Biochemistry·A D Malcolm
Nov 1, 1970·European Journal of Biochemistry·M RippaP Fasella
Apr 1, 1980·Annals of Neurology·A PlaitakisR J Desnick
Mar 15, 1977·European Journal of Biochemistry·M Nemat-Gorgani, G Dodd
May 1, 1982·Neurochemical Research·T YamaguchiS Maruyama
May 1, 1975·CRC Critical Reviews in Biochemistry·N J Greenfield
Sep 1, 1970·European Journal of Biochemistry·A D Malcolm, G K Radda
Sep 15, 1975·European Journal of Biochemistry·R VenardM Iwatsubo
Feb 10, 1971·Biochimica Et Biophysica Acta·T Yamaguchi
May 1, 1969·Biochimica Et Biophysica Acta·P J Anderson, P Johnson
Nov 1, 1970·Archives of Biochemistry and Biophysics·M H Stryker, F S Parker
Aug 1, 1979·Archives of Biochemistry and Biophysics·K G Gould, P C Engel
May 25, 1967·Biochemical and Biophysical Research Communications·G H Dodd, G K Radda
Jan 6, 2009·International Journal of Biological Macromolecules·Marjan SabbaghianMohsen Nemat-Gorgani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.