PMID: 9636229Jun 24, 1998Paper

Conformational changes couple Na+ and glucose transport

Proceedings of the National Academy of Sciences of the United States of America
D D LooE M Wright

Abstract

The mechanism by which cotransport proteins couple their substrates across cell membranes is not known. A commonly proposed model is that cotransport results from ligand-induced conformational transitions that change the accessibility of ligand-binding sites from one side of the membrane to the other. To test this model, we have measured the accessibility of covalent probes to a cysteine residue (Q457C) placed in the putative sugar-translocation domain of the Na+/glucose cotransporter (SGLT1). The mutant protein Q457C was able to transport sugar, but transport was abolished after alkylation by methanethiosulfonate reagents. Alkylation blocked sugar translocation but not sugar binding. Accessibility of Q457C to alkylating reagents required external Na+ and was blocked by external sugar and phlorizin. The voltage dependence of accessibility was directly correlated with the presteady-state charge movement of SGLT1. Voltage-jump experiments with rhodamine-6-maleimide-labeled Q457C showed that the time course and level of changes in fluorescence closely followed the presteady-state charge movement. We conclude that conformational changes are responsible for the coupling of Na+ and sugar transport and that Q457 plays a critical role ...Continue Reading

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Citations

Jul 12, 2005·The Journal of Membrane Biology·S EskandariD D F Loo
Jul 2, 2008·The Journal of Membrane Biology·Donald D F LooErnest M Wright
Mar 9, 2011·Pflügers Archiv : European journal of physiology·Chiara GhezziIan C Forster
Apr 30, 2010·Journal of Physiology and Biochemistry·Carmen Castaneda-Sceppa, Francisco Castaneda
Dec 3, 2003·Best Practice & Research. Clinical Gastroenterology·Ernest M WrightEric Turk
Oct 6, 2001·British Journal of Pharmacology·B A HirayamaE M Wright
Mar 30, 2001·The Journal of Biological Chemistry·M J RouxS Supplisson
Apr 14, 2009·The Journal of Biological Chemistry·Anne-Kristine MeinildNanna MacAulay
Dec 29, 2010·The Journal of Biological Chemistry·Ana Díez-Sampedro, Stephanie Barcelona
Dec 15, 2004·The Journal of General Physiology·Donald D F LooErnest M Wright
Feb 1, 2006·The Journal of General Physiology·Dominique G GagnonJean-Yves Lapointe
Nov 30, 2006·The Journal of General Physiology·Donald D F LooErnest M Wright
Sep 26, 2012·The Journal of General Physiology·Jean-Philippe LongpréJean-Yves Lapointe
Jan 11, 2001·Protein Science : a Publication of the Protein Society·K DoringF Jähnig
Oct 25, 2006·Annual Review of Physiology·Louis J DeFelice, Tapasree Goswami
Dec 14, 2011·American Journal of Physiology. Cell Physiology·Monica Sala-RabanalErnest M Wright
May 2, 2008·American Journal of Physiology. Cell Physiology·Tiemin LiuMel Silverman
Aug 22, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasunobu Suketa
Sep 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ana Diez-SampedroHermann Koepsell
Sep 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Adiel CohenNathan Nelson
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·S EskandariG A Zampighi
Mar 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·H Peter LarssonMichael P Kavanaugh
Apr 22, 2006·The Journal of Physiology·Monica Sala-RabanalErnest M Wright
Jan 16, 2007·Journal of Internal Medicine·E M WrightD F Loo
Jun 25, 2002·Biophysical Journal·Ming Li, Henry A Lester
May 21, 2005·Biochimica Et Biophysica Acta·Dominique G GagnonJean-Yves Lapointe
Apr 20, 2011·Molecular Plant·Dietmar Geiger
Mar 27, 2010·Biophysical Journal·Jean-Philippe LongpréJean-Yves Lapointe
Dec 31, 2010·Biophysical Journal·Jean-Philippe Longpré, Jean-Yves Lapointe
May 29, 2003·Biophysical Journal·Daniel Krofchick, Mel Silverman

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