PMID: 38111Jun 1, 1979

Conformational changes in bovine-liver glutamate dehydrogenase: a spin-label study

European Journal of Biochemistry
A ZantemaG T Robillard

Abstract

A spin-labelled analogue of p-chloromercuribenzoate reacts specifically with glutamate dehydrogenase. The most marked change in the properties of the spin-labelled enzyme is a fivefold decrease in the rate of reduction of the coenzyme by L-glutamate and no change in the rate of oxidation by 2-oxoglutarate. The electron spin resonance spectrum is a sensitive probe for the conformational state of the enzyme. Spin-labelled glutamate dehydrogenase in the presence of saturating concentrations of NADPH and 2-oxoglutarate or L-glutamate shows a complete conformational change while in the presence of NADP+ and 2-oxoglutarate only half of the protomers have changed conformation. The conformational change upon addition of NADPH to the spin-labelled glutamate dehydrogenase in the presence of 2-oxoglutarate happens in a concerted way between 20 and 80% saturation with NADPH. One of the conformations is favoured by the activator ADP while the other is favoured by the inhibitor GTP.

References

Feb 7, 1977·Biochemical and Biophysical Research Communications·J M JallonL Piette
Sep 15, 1977·European Journal of Biochemistry·H Koekoek, G T Robillard
Apr 1, 1978·European Journal of Biochemistry·M P CossonM Iwatsubo
Jan 3, 1977·European Journal of Biochemistry·A ZantemaG T Robillard
Jan 1, 1978·Molecular Biology, Biochemistry, and Biophysics·A Levitzki
Jun 21, 1976·Biochemical and Biophysical Research Communications·B E TilleyP F Hall
Oct 18, 1976·Biochemical and Biophysical Research Communications·M P CossonJ C Talbot
Nov 1, 1972·The Biochemical Journal·G Melzi d'Eril, K Dalziel
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J C Boeyens, H M McConnell
Sep 19, 1972·Biochimica Et Biophysica Acta·R A ProughH F Fisher

Related Concepts

Bos indicus
Chloromercuribenzoates
Glutamate Dehydrogenase
Liver
NADP
In Vivo NMR Spectroscopy
Protein Conformation
Spin Labels

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