Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation

The Journal of Biological Chemistry
Soon Goo Lee, Joseph M Jez

Abstract

Phosphocholine (pCho) is a precursor for phosphatidylcholine and osmoprotectants in plants. In plants, de novo synthesis of pCho relies on the phosphobase methylation pathway. Phosphoethanolamine methyltransferase (PMT) catalyzes the triple methylation of phosphoethanolamine (pEA) to pCho. The plant PMTs are di-domain methyltransferases that divide the methylation of pEA in one domain from subsequent methylations in the second domain. To understand the molecular basis of this architecture, we examined the biochemical properties of three Arabidopsis thaliana PMTs (AtPMT1-3) and determined the X-ray crystal structures of AtPMT1 and AtPMT2. Although each isoform synthesizes pCho from pEA, their physiological roles differ with AtPMT1 essential for normal growth and salt tolerance, whereas AtPMT2 and AtPMT3 overlap functionally. The structures of AtPMT1 and AtPMT2 reveal unique features in each methyltransferase domain, including active sites that use different chemical mechanisms for phosphobase methylation. These structures also show how rearrangements in both the active sites and the di-domain linker form catalytically competent active sites and provide insight on the evolution of the PMTs in plants, nematodes, and apicomplexans....Continue Reading

References

Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Jan 1, 1995·Annual Review of Biochemistry·C Kent
Sep 17, 1996·Biochemistry·Z FuF Takusagawa
Nov 25, 1997·Biochimica Et Biophysica Acta·M I Kanipes, S A Henry
Mar 30, 2000·Protein Science : a Publication of the Protein Society·W Dall'Acqua, P Carter
May 4, 2001·Trends in Plant Science·T Munnik
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Andrew D Hanson, Sanja Roje
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·S D McNeilA D Hanson
Jul 16, 2003·Biochemistry·Yoshimi TakataFusao Takusagawa
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gabriella PessiChoukri Ben Mamoun
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Oct 26, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Chunjiang YeBin Wang
Nov 1, 1988·Plant Physiology·A H Datko, S H Mudd
Dec 1, 1988·Plant Physiology·A H Datko, S H Mudd
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Oct 5, 2006·Journal of the American Chemical Society·Yansheng ZhangOliver Yu
Mar 3, 2007·Annual Review of Biochemistry·Chaitan KhoslaDavid E Cane
Nov 21, 2007·Nature·Katherine A Henzler-WildmanDorothee Kern
Dec 14, 2007·Nature·Katherine Henzler-Wildman, Dorothee Kern
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Jul 24, 2010·The Journal of Biological Chemistry·Michael D BeGoraElizabeth A Weretilnyk
Dec 2, 2011·Journal of the American Chemical Society·Yechun WangJoseph M Jez
Jul 10, 2012·Bioorganic & Medicinal Chemistry Letters·Soon Goo LeeJoseph M Jez
Apr 2, 2014·Trends in Parasitology·Soon Goo Lee, Joseph M Jez

❮ Previous
Next ❯

Citations

Sep 16, 2018·The Plant Journal : for Cell and Molecular Biology·Yu-Chi LiuYuki Nakamura
Dec 31, 2017·The Journal of Biological Chemistry·Joseph J Barycki
Aug 11, 2019·The Plant Journal : for Cell and Molecular Biology·Anh H NgoYuki Nakamura
Mar 11, 2018·Biochemical and Biophysical Research Communications·Chao-Yuan YuKazue Kanehara
Jun 8, 2021·The Plant Journal : for Cell and Molecular Biology·Martin TannertMargret Köck
Nov 24, 2021·Journal of the American Chemical Society·Sei-Hyun ChoiHung-Wen Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.