Conformational changes in the extrinsic manganese stabilizing protein can occur upon binding to the photosystem II reaction center: an isotope editing and FT-IR study

Biochemistry
R S HutchisonBridgette A Barry

Abstract

Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II reaction center. Extraction is associated with decreased stability of manganese binding by the enzyme and by loss in high rates of oxygen evolution activity; reconstitution reverses these phenomena. Since little is known about the assembly of complex membrane proteins, we have employed isotope editing and vibrational spectroscopy to obtain information about any changes in secondary structure that occur in MSP upon functional reconstitution to photosystem II. The spectroscopic data obtained are consistent with substantial changes in conformation when MSP binds to photosystem II; approximately 30-40% of the peptide backbone undergoes a change in secondary structure. These conclusions were reached by comparing different aliquots, before and after binding, of the same 13[C]MSP sample. Analysis of amide I band line shapes through Fourier deconvolution and nonlinear regression suggests that binding of MSP to photosystem ...Continue Reading

References

Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·M Jackson, H H Mantsch

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Citations

Apr 19, 2013·Journal of the American Chemical Society·Adam R OffenbacherBridgette A Barry
Oct 3, 2012·PloS One·Irma N RobertsChristiane Funk
Mar 13, 2014·The Journal of Physical Chemistry. B·Adam R OffenbacherBridgette A Barry
Nov 15, 2006·The Journal of Chemical Physics·Kenan GündoğduChristopher M Cheatum
Jun 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shuqin LiTingyun Kuang
Feb 15, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hana Popelkova, Charles F Yocum
Sep 13, 2005·Journal of Photochemistry and Photobiology. B, Biology·Guangye HanTingyun Kuang
Mar 3, 2004·Biophysical Journal·Bengt SvenssonBridgette A Barry
Apr 5, 2014·Chemical Reviews·Macarena Marín, Thomas Ott
Jul 5, 2013·The Journal of Physical Chemistry. B·Adam R OffenbacherBridgette A Barry
Mar 21, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Coral Del Val, Ana-Nicoleta Bondar
Apr 30, 2002·Plant & Cell Physiology·Akihiko TohriIsao Enami
Mar 20, 2010·The Journal of Biological Chemistry·Elizabeth D KimSunyoung Kim
Aug 2, 2017·Accounts of Chemical Research·Bridgette A BarryZhanjun Guo

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