PMID: 2497777Mar 7, 1989Paper

Conformational changes of phospholipid headgroups induced by a cationic integral membrane peptide as seen by deuterium magnetic resonance

Biochemistry
M RouxM Bloom

Abstract

Deuterium nuclear magnetic resonance (2H NMR) was used to study the interaction of a cationic amphiphilic peptide with pure DMPC membranes and with mixed bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylserine (DMPS). The choline and serine headgroups were selectively deuteriated at the alpha and beta positions. The amphiphilic peptide, with 20 leucine residues in the hydrophobic core and two cationic hydrophilic lysine residues at each end, spanned the lipid bilayer. Although 2H NMR experiments using DMPC with perdeuteriated fatty acyl chains showed that the average order parameter of the hydrophobic region was not significantly modified by the incorporation of the amphiphilic peptide, for either DMPC or DMPC/DMPS (5:1) bilayers, large perturbations of the quadrupolar splittings of the choline and serine headgroups were observed. The results obtained with the DMPC headgroup suggest that the incorporation of the cationic peptide in both DMPC and DMPC/DMPS (5:1) bilayers leads to a structural perturbation directly related to the net charge on the membrane surface. The magnitude of the observed effect seems to be similar to those observed previously with other cationic molecules [Seelig, J., MacDonald,...Continue Reading

References

Dec 1, 1987·Biochemistry·J SeeligP G Scherer
Jan 1, 1985·European Biophysics Journal : EBJ·K P PaulsR S Hodges
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F Sixl, A Watts
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Citations

Feb 8, 2008·Journal of Fluorescence·Agnieszka OlzyńskaMartin Hof
Nov 1, 2008·Cell Biochemistry and Biophysics·Roberth ByströmGerhard Gröbner
Jan 1, 1991·The International Journal of Biochemistry·T Cserháti, M Szögyi
May 16, 2000·Chemistry and Physics of Lipids·M Langner, K Kubica
May 16, 2000·Solid State Nuclear Magnetic Resonance·P M MacdonaldD Semchyschyn
Oct 29, 2015·The Journal of Physical Chemistry. B·Alexandru BotanJoona Petteri Tynkkynen
Mar 27, 2007·Biophysical Chemistry·Valeriya M IoffeAleksey Vasilev
Mar 1, 2014·The Journal of Membrane Biology·Monika Naumowicz, Zbigniew Artur Figaszewski
Nov 9, 1992·Biochimica Et Biophysica Acta·J R Rydall, P M Macdonald
Aug 29, 2003·Biophysical Journal·Nola FullerR Peter Rand
Sep 22, 2012·Biophysical Journal·Jacques P F DouxJ Antoinette Killian
Jan 9, 1990·Biochemistry·G Beschiaschvili, J Seelig
Feb 4, 1992·Biochemistry·J R Rydall, P M Macdonald
Jul 20, 1999·The Journal of Biological Chemistry·M R de PlanqueJ A Killian
Oct 28, 2003·Molecular Membrane Biology·Maurits R R de Planque, J Antoinette Killian

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