Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis

FEBS Letters
Ariel E MechalyMarco Bellinzoni

Abstract

rv1098c, an essential gene in Mycobacterium tuberculosis, codes for a class II fumarase. We describe here the crystal structure of Rv1098c in complex with l-malate, fumarate or the competitive inhibitor meso-tartrate. The models reveal that substrate binding promotes the closure of the active site through conformational changes involving the catalytic SS-loop and the C-terminal domain, which likely represents a general feature of this enzyme superfamily. Analysis of ligand-enzyme interactions as well as site-directed mutagenesis suggest Ser318 as one of the two acid-base catalysts.

References

Apr 28, 1988·Biochimica Et Biophysica Acta·S A WoodsJ R Guest
Aug 1, 1995·Nature Structural Biology·T M WeaverL J Banaszak
Apr 1, 1997·Protein Science : a Publication of the Protein Society·T WeaverL Banaszak
Mar 27, 2003·Molecular Microbiology·Christopher M SassettiEric J Rubin
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Irwin A Rose, Todd M Weaver
Oct 6, 2005·Acta Crystallographica. Section D, Biological Crystallography·Todd Weaver
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Tomoko GendaHachiro Ozaki
Jan 24, 2007·Biochimica Et Biophysica Acta·Mario BarteriSimona Rotella
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Jun 15, 2011·Biochemistry·Guntur FibriansahAndy-Mark W H Thunnissen
Dec 9, 2010·ACS Medicinal Chemistry Letters·Katrin Stierand, Matthias Rarey

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Citations

Feb 3, 2015·Biotechnology Advances·Bi-Shuang ChenUlf Hanefeld
Apr 6, 2013·Current Opinion in Chemical Biology·Matthew M HeberlingDick B Janssen
Jun 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Monica KasbekarCraig J Thomas
Feb 15, 2019·The FEBS Journal·Mariana A Ajalla AleixoMaria Cristina Nonato
Dec 22, 2019·Biochimie·Raymond S Ochs, Tanaji T Talele
Feb 8, 2021·International Journal of Biological Macromolecules·Iara Aimê CardosoMaria Cristina Nonato
Jun 25, 2015·Microbiology Spectrum·Anthony D Baughn, Kyu Y Rhee

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