Conformational Complexity in the LH2 Antenna of the Purple Sulfur Bacterium Allochromatium vinosum Revealed by Hole-Burning Spectroscopy

The Journal of Physical Chemistry. a
Adam KellRyszard Jankowiak

Abstract

This work discusses the protein conformational complexity of the B800-850 LH2 complexes from the purple sulfur bacterium Allochromatium vinosum, focusing on the spectral characteristics of the B850 chromophores. Low-temperature B850 absorption and the split B800 band shift blue and red, respectively, at elevated temperatures, revealing isosbestic points. The latter indicates the presence of two (unresolved) conformations of B850 bacteriochlorophylls (BChls), referred to as conformations 1 and 2, and two conformations of B800 BChls, denoted as B800Rand B800B. The energy differences between average site energies of conformations 1 and 2, and B800Rand B800Bare similar (∼200 cm-1), suggesting weak and strong hydrogen bonds linking two major subpopulations of BChls and the protein scaffolding. Although conformations 1 and 2 of the B850 chromophores, and B800Rand B800B, exist in the ground state, selective excitation leads to 1 → 2 and B800R→ B800Bphototransformations. Different static inhomogeneous broadening is revealed for the lowest energy exciton states of B850 (fwhm ∼195 cm-1) and B800R(fwhm ∼140 cm-1). To describe the 5 K absorption spectrum and the above-mentioned conformations, we employ an exciton model with dichotomous pro...Continue Reading

References

Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Clemens HofmannJürgen Köhler
Oct 9, 2007·Biophysical Journal·Julius JanusonisRienk van Grondelle
Apr 16, 2009·The Journal of Physical Chemistry. B·O ZerlauskieneL Valkunas
Jan 29, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Mihkel PajusaluArvi Freiberg
Jun 8, 2012·Standards in Genomic Sciences·Thomas WeissgerberChristiane Dahl
Aug 23, 2012·The Journal of Physical Chemistry. B·Ralf KunzJürgen Köhler
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Liam ClearyJianshu Cao
May 31, 2013·The Journal of Physical Chemistry. B·Adam KellRyszard Jankowiak
Jun 20, 2014·The Journal of Physical Chemistry. B·Olga Rancova, Darius Abramavicius
Aug 26, 2014·Photosynthesis Research·Alexander LöhnerJürgen Köhler
Feb 10, 2016·Scientific Reports·Marco FerrettiRienk van Grondelle
Jul 4, 2016·Biochimica Et Biophysica Acta·Jeffrey R ReimersElmars Krausz

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Citations

Mar 1, 2018·The Journal of Physical Chemistry Letters·Marco SchröterDonatas Zigmantas
Jul 10, 2018·The Journal of Physical Chemistry Letters·Adam KellRyszard Jankowiak

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